PKA C-gamma Antibody (2E4)

Novus Biologicals | Catalog # NBP2-37487

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E4
View all PKA C-gamma Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PKA C-gamma expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA C-gamma Antibody (2E4)

Western Blot: PKA C-gamma Antibody (2E4)BSA Free [NBP2-37487]

Western Blot: PKA C-gamma Antibody (2E4)BSA Free [NBP2-37487]

Western Blot: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis using PRKACG mAb against human PRKACG (AA: 164-351) recombinant protein. (Expected MW is 47.1 kDa)
Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis of rectum cancer tissues using PRKACG mouse mAb with DAB staining.
Flow Cytometry: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

Flow Cytometry: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

Flow Cytometry: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis of MCF-7 cells using PRKACG mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis of liver cancer tissues using PRKACG mouse mAb with DAB staining.
ELISA: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

ELISA: PKA C-gamma Antibody (2E4) - BSA Free [NBP2-37487]

ELISA: PKA C-gamma Antibody (2E4) [NBP2-37487] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PKA C-gamma Antibody (2E4)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 3 using NBP2-37487 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKA C-gamma

Long Name

cAMP-dependent protein kinase catalytic subunit gamma

Alternate Names

EC 2.7.11.11, PRKACG

Gene Symbol

PRKACG

Additional PKA C-gamma Products

Product Documents for PKA C-gamma Antibody (2E4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PKA C-gamma Antibody (2E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PKA C-gamma Antibody (2E4) (1)

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Customer Images

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  • PKA C-gamma Antibody (2E4)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult lung
    Species: Mouse
    Verified Customer | Posted 11/04/2019
    Protein content from mouse primary endothelial cells was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody PKA C-gamma diluted 1:1000 in 5% BSA 2, before incubation with anti mouse secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
    PKA C-gamma Antibody (2E4) NBP2-37487

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Protocols

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FAQs

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