PKC delta Antibody (K15-K)

Novus Biologicals | Catalog # NBP1-30125

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # K15-K
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Product Specifications

Immunogen

Peptide derived from N-terminal sequence of human PKC delta. Antibody recognizes the epitope located between Lys42 - Trp55

Epitope

Lys42 - Trp55

Specificity

This antibody is specific for PKC-delta.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for PKC delta Antibody (K15-K)

Western Blot: PKC delta Antibody (K15-K) [NBP1-30125]

Western Blot: PKC delta Antibody (K15-K) [NBP1-30125]

Western Blot: PKC delta Antibody (K15-K) [NBP1-30125] - Lane 1-100 ng; lane 2-200ng; lane 3-500 ng of human recombinant N-term GST PKC-delta; MW=104kDa.

Applications for PKC delta Antibody (K15-K)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
Dilutions for IP must be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC delta

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca2+. On the other hand, nPKC delta and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Long Name

Protein Kinase C delta

Alternate Names

PRKCD

Entrez Gene IDs

5580 (Human)

Gene Symbol

PRKCD

UniProt

Additional PKC delta Products

Product Documents for PKC delta Antibody (K15-K)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PKC delta Antibody (K15-K)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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