PKC delta [p Tyr311] Antibody - BSA Free

Name: PKC delta [p Tyr311] Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP3-05431
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-05431

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y311 of rat PKC delta (NP_579841.1). VGIYQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC delta [p Tyr311] Antibody - BSA Free

Western Blot: PKC delta [p Tyr311] AntibodyBSA Free [NBP3-05431]

Western Blot: PKC delta [p Tyr311] Antibody [NBP3-05431] - Western blot analysis of extracts of U937 and Raw264.7 cells, using PKC delta antibody (NBP3-05431) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Applications for PKC delta [p Tyr311] Antibody - BSA Free

Application

Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKC delta

PKC-delta has a biological activity which is closely related to Pkc1, and PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC-delta is required for binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). It has been reported a mechanism for the regulation of peripheral B-cell survival by serine/threonine protein kinase Cdelta (PKC-delta): spontaneous death of resting B cells is regulated by nuclear localization of PKC-delta that contributes to phosphorylation of histone H2B at serine 14 (S14-H2B) (3).

Long Name

Protein Kinase C delta

Alternate Names

PRKCD

Gene Symbol

PRKCD

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Product Specific Notices for PKC delta [p Tyr311] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.