PKLR Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-56319

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PKLR Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to PKLR(pyruvate kinase, liver and RBC) The peptide sequence was selected from the N terminal of PKLR (NP_870986). Peptide sequence STSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVRE. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22367961).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PKLR Antibody - BSA Free

Western Blot: PKLR Antibody [NBP1-56319]

Western Blot: PKLR Antibody [NBP1-56319]

Western Blot: PKLR Antibody [NBP1-56319] - HepG2 cell lysate, concentration 1.0ug/ml.
Immunohistochemistry-Paraffin: PKLR Antibody [NBP1-56319]

Immunohistochemistry-Paraffin: PKLR Antibody [NBP1-56319]

Immunohistochemistry-Paraffin: PKLR Antibody [NBP1-56319] - Human kidney lysate tissue at an antibody concentration of 4-8ug/ml.

Applications for PKLR Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKLR

PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Long Name

Pyruvate Kinase, Liver And RBC

Alternate Names

PK1, PKL, Pyruvate Kinase 1, RPK

Entrez Gene IDs

5313 (Human)

Gene Symbol

PKLR

UniProt

P30613-2 (Human)

Additional PKLR Products

Product Documents for PKLR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PKLR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PKLR Antibody - BSA Free

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Protocols

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