PKLR Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-58892

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PKLR Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGA

Reactivity Notes

Mouse 86%, Rat 87%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit PKLR Antibody - BSA Free (NBP2-58892) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PKLR Antibody - BSA Free

Western Blot: PKLR Antibody [NBP2-58892]

Western Blot: PKLR Antibody [NBP2-58892]

Western Blot: PKLR Antibody [NBP2-58892] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: PKLR Antibody [NBP2-58892]

Immunocytochemistry/ Immunofluorescence: PKLR Antibody [NBP2-58892]

Immunocytochemistry/Immunofluorescence: PKLR Antibody [NBP2-58892] - Staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus. Antibody staining is shown in green.
Western Blot: PKLR Antibody [NBP2-58892]

Western Blot: PKLR Antibody [NBP2-58892]

Western Blot: PKLR Antibody [NBP2-58892] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.

Applications for PKLR Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKLR

The protein encoded by the Pyruvate Kinase gene is a pyruvate kinase that catalyzes the transphosphorylation of phosphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Long Name

Pyruvate Kinase, Liver And RBC

Alternate Names

PK1, PKL, Pyruvate Kinase 1, RPK

Gene Symbol

PKLR

Additional PKLR Products

Product Documents for PKLR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PKLR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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