PLA2G1B Antibody

Novus Biologicals | Catalog # NBP1-06063

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PLA2G1B Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence NKAHKNLDTKKYCQS corresponding to C-Terminus according to NP_000919.1.

Epitope

NKAHKNLDTKKYCQS

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PLA2G1B Antibody

Western Blot: PLA2G1B Antibody [NBP1-06063]

Western Blot: PLA2G1B Antibody [NBP1-06063]

Western Blot: PLA2G1B Antibody [NBP1-06063] - HEK293 overexpressing PLA2G1B and probed with (mock transfection in first lane).
Western Blot: PLA2G1B Antibody [NBP1-06063]

Western Blot: PLA2G1B Antibody [NBP1-06063]

Western Blot: PLA2G1B Antibody [NBP1-06063] - (0.01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PLA2G1B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01 ug/ml
Application Notes
WB: Approx. 17-18 kDa band observed in human pancreas lysates (calculated MW of 16.4 kDa band according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx. 18 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLA2G1B

Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]).[supplied by OMIM]

Long Name

Phospholipase A2 Group IB

Alternate Names

PLA2, PLA2A, PPLA2

Entrez Gene IDs

5319 (Human); 18778 (Mouse); 29526 (Rat)

Gene Symbol

PLA2G1B

UniProt

NP_000919.1 (Human)

Additional PLA2G1B Products

Product Documents for PLA2G1B Antibody

Certificate of Analysis

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Product Specific Notices for PLA2G1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PLA2G1B Antibody

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