PLAG1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56656

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PLAG1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EPVDFLDPFTCNVSVPIKDELLPVMSLPSSELLSKPFTNTLQLNLYNTPFQSMQSSGSAHQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLAG1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PLAG1 Antibody [NBP2-56656]

Immunocytochemistry/ Immunofluorescence: PLAG1 Antibody [NBP2-56656]

Immunocytochemistry/Immunofluorescence: PLAG1 Antibody [NBP2-56656] - Staining of human cell line U-2 OS shows localization to nuclear speckles.
PLAG1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: PLAG1 Antibody - BSA Free [NBP2-56656]

Chromatin Immunoprecipitation-exo-Seq: PLAG1 Antibody - BSA Free [NBP2-56656]

ChIP-Exo-Seq composite graph for Anti-PLAG1 (NBP2-56656) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for PLAG1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLAG1

Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

COL1A2/PLAG1 fusion, pleiomorphic adenoma gene 1, Pleiomorphic adenoma gene 1 protein, SGPA, zinc finger protein PLAG1, ZNF912

Gene Symbol

PLAG1

Additional PLAG1 Products

Product Documents for PLAG1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PLAG1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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