Plasma Cell Marker Antibody (SPM310)

Novus Biologicals | Catalog # NBP2-34359

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Key Product Details

Species Reactivity

Human, Rat (Negative)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPM310
View all Plasma Cell Marker Primary Antibodies »

Product Specifications

Immunogen

Pancreatic cancer-related mucin

Reactivity Notes

Does not react with Rat.

Localization

Cytoplasmic

Marker

Plasma Cell Marker

Specificity

It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This monoclonal antibody superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this monoclonal antibody is not suitable for staining frozen tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34407)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Plasma Cell Marker Antibody (SPM310)

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359]

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359]

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] - Human tonsil tissue. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.
Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359]

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359]

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] - Formalin-fixed paraffin-embedded human tonsil stained with plasma cell marker Monoclonal antibody (SPM310)
Plasma Cell Marker Antibody (SPM310)

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] -

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] - Formalin-paraffin human tonsil stained with Plasma Cell Marker MAb (SPM310).
Plasma Cell Marker Antibody (SPM310)

Immunohistochemistry: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] -

The results of immunohistochemistry (IHC). In total of 29 patients, 15 were diagnosed with liposarcoma (Metastasis occurred in eight patients during follow-up) and 14 were diagnosed with leiomyosarcoma (Metastasis occurred in nine patients during follow-up). Proteins of PAX5 and LTB were shown to be significantly down-regulated in the tumor cells of primary sarcomas with metastasis while the markers of B cells (CD19 and CD 38) were not detected in almost all primary tumor (a). Although CD19 and CD 38 were found in lymph node, as the advice of a pathologist in our hospital, these were not surprising as B cells are abundant in lymph node. Therefore, the IHC results of CD19 and CD 38 did not prove or disprove the hypothesis. Furthermore, in the absence of a good CD19 and CD38 antibody, we considered CD138 (Syndean-1) and SPM310 plasma cell marker antibody (Novus NBP2-34359) as other proven plasma cell markers. However, only four of 29 sarcomas were found plasma cells in the HE staining section of the tumor and none of the four patients had metastases (b). This might be because tumor-infiltrating immune cells tended to be located around the tumor rather than within it, and sarcomas tended to be excised en bloc, so there were no paracancer tissues as a control. Fortunately, the results of Mann–Whitney U test suggested that PAX5 (P < 0.001), LTB (P < 0.001) in were all highly expressed in well differentiated primary sarcomas and primary sarcomas without metastasis (c, d) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33397394), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Plasma Cell Marker Antibody (SPM310)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Note that this antibody is not suitable for staining frozen tissues.

Reviewed Applications

Read 1 review rated 4 using NBP2-34359 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Product Documents for Plasma Cell Marker Antibody (SPM310)

Certificate of Analysis

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Product Specific Notices for Plasma Cell Marker Antibody (SPM310)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Plasma Cell Marker Antibody (SPM310)

Customer Reviews for Plasma Cell Marker Antibody (SPM310) (1)

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  • Plasma Cell Marker Antibody (SPM310)
    Name: Santhosh Sivajothi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 05/18/2018
    Heat induced antigen retrieval was performed in citrate buffer (pH 6) at 95C for 20 minutes.
    Plasma Cell Marker Antibody (SPM310) NBP2-34359

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