PLC-gamma 1 Antibody (2F3D11) - BSA Free

Name: PLC-gamma 1 Antibody (2F3D11) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-61790
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-61790

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 2F3D11

Format

BSA Free

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Product Specifications

Immunogen

Purified recombinant fragment of human PLC-gamma 1 (AA: 39-181) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

148.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLC-gamma 1 Antibody (2F3D11) - BSA Free

Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 39-181)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mAb against human PLCG1 (AA: 39-181) recombinant protein. (Expected MW is 43 kDa)

Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mouse mAb against Jurkat (1), K562 (2), A431 (3), Hela (4), and PC-12 (5) cell lysate.

ELISA: PLC-gamma 1 Antibody (2F3D11) - BSA Free [NBP2-61790]

ELISA: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: PLC-gamma 1 Antibody (2F3D11) - BSA Free [NBP2-61790]

ELISA: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis of Hela cells using PLCG1 mouse mAb (green) and negative control (red).

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Applications for PLC-gamma 1 Antibody (2F3D11) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 1

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.;

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Gene Symbol

PLCG1

Additional PLC-gamma 1 Products

Product Documents for PLC-gamma 1 Antibody (2F3D11) - BSA Free

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Certificate of Analysis

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Product Specific Notices for PLC-gamma 1 Antibody (2F3D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.