PLC-gamma 1 [p Ser1248] Antibody

Name: PLC-gamma 1 [p Ser1248] Antibody
Brand: Novus Biologicals
SKU: NBP3-13252
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-13252

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all PLC-gamma 1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1248 of human PLC-gamma 1. The exact sequence is proprietary.

Modification

p Ser 1248

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PLC-gamma 1 [p Ser1248] Antibody

Western Blot: PLC-gamma 1 [p Ser1248] Antibody [NBP3-13252]

Western Blot: PLC-gamma 1 [p Ser1248] Antibody [NBP3-13252] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-gamma 1 (phospho Ser1248) antibody (NBP3-13252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: PLC-gamma 1 [p Ser1248] Antibody [NBP3-13252] -

Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG1 (phospho Ser1248) antibody (NBP3-13252) diluted at 1:1000 and PLCG1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: PLC-gamma 1 [p Ser1248] Antibody [NBP3-13252] -

Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG1 (phospho Ser1248) antibody (NBP3-13252) diluted at 1:1000 and PLCG1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PLC-gamma 1 [p Ser1248] Antibody

Application

Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 1

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry (3).

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Gene Symbol

PLCG1

Additional PLC-gamma 1 Products

Product Documents for PLC-gamma 1 [p Ser1248] Antibody

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Certificate of Analysis

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Product Specific Notices for PLC-gamma 1 [p Ser1248] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.