PLEKHS1 Antibody (3B6) - Azide and BSA Free

Western Blot: PLEKHS1 Antibody (3B6) [H00079949-M07]

Western Blot: C10orf81 Antibody (3B6) [H00079949-M07] - Analysis of C10orf81 expression in Jurkat (Cat # L017V1).

Western Blot: PLEKHS1 Antibody (3B6) - Azide and BSA Free [H00079949-M07] -

PLEKHS1 is over-expressed in thyroid cancer (TC)-derived cell lines and primary tumors. (A) PLEKHS1 expression in TC cell lines. Left and right: PLEKHS1 mRNA and protein expression levels as determined using qPCR and immunoblotting, respectively. The quantification results (band intensity) in the right panel are shown in the left panel. (B) Differential expression of PLEKHS1 mRNA between non-cancerous adjacent thyroid tissues and primary PTC tumors. The TCGA cohort of PTC tumors and adjacent tissues are analyzed and mRNA levels were expressed as FPKM (Fragments Per Kilobase Million). (C) Higher levels of PLEKHS1 expression in ATC tumors than PTCs. (D) PLEKHS1 mRNA and protein expression levels in 20 PTC tumors as determined using qPCR and immunoblotting, respectively. The quantification results (band intensity) in the bottom panel are shown in the top panel. (E) Positive correlation between PLEKHS1 mRNA and protein expression, as revealed by the analysis of combined results from both TC cell lines (A) and primary PTC tumors (D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32752127), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PLEKHS1 Antibody (3B6) - Azide and BSA Free [H00079949-M07] -

PLEKHS1 over-expression promotes AKT phosphorylation, proliferation and migration/invasion in PTC- and ATC-derived cells. (A) Increased AKT phosphorylation in PLEKHS1-transfected cells. MDA-T32, MDA-T41, U-hth-74, and U-hth-104 cells were transfected with control (Con) empty and PLEKHS1 (PLEK) expression vectors, respectively, and harvested 48 h post-transfection for total AKT and phosphorylated AKT levels using immunoblotting. One representative experiment out of three independent experiments is shown. The fold change for PLEKHS1, total and phosphorylated AKT is indicated (based on their signal intensity normalized to beta -actin). (B) Increased cell proliferation of cells overexpressing PLEKHS1. MDA-T32, MDA-T41 and U-hth-104 cells above were incubated for 48 h and then counted. The number of control cells was set as 100%. Shown are the results from three independent experiments. (C) Enhanced migration and invasion of PLEKHS1-overexpressed cells. MDA-T32, MDA-T41 and U-hth-104 cells above were analyzed for their migration and invasion capacities using a transwell assay system. Left: representative images of migration or invasion. Right: the quantification of migrating or invading cells based on three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32752127), licensed under a CC-BY license. Not internally tested by Novus Biologicals.