Mouse PLGF ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-49879

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.25 pg/mL

Assay Range

3.91 - 250 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse PLGF in samples. No significant cross-reactivity or interference between Mouse PLGF and analogues was observed.

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% 10

Inter-Assay Precision (Precision between assays) CV% 10

Recovery for Mouse PLGF ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Mouse PLGF ELISA Kit (Colorimetric)
Mouse PLGF ELISA Kit (Colorimetric)

Scientific Data Images for Mouse PLGF ELISA Kit (Colorimetric)

Mouse PLGF ELISA Kit (Colorimetric)

ELISA: Mouse PLGF ELISA Kit (Colorimetric) [NBP3-49879]

Standard Curve Reference.

Kit Contents for Mouse PLGF ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PlGF

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Gene Symbol

PGF

Additional PlGF Products

Product Documents for Mouse PLGF ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse PLGF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Mouse PLGF ELISA Kit (Colorimetric)

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  • Q: Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?

    A: Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.

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