Human PlGF Quantikine ELISA Kit

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Human PlGF ELISA Cell Culture Supernate Standard Curve
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Human PlGF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Sensitivity
7 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human PlGF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human PlGF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human PlGF in cell culture supernates, serum, plasma, and urine. The immunoassay kit contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

Recovery

The recovery of PlGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 97 82-107
Citrate Plasma (n=5) 99 93-120
EDTA Plasma (n=5) 102 92-117
Heparin Plasma (n=5) 94 81-113
Serum (n=5) 96 88-118
Urine (n=4) 97 85-112

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of PlGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human PlGF ELISA Linearity

Scientific Data

Human PlGF ELISA Cell Culture Supernate Standard Curve

Human PlGF ELISA Serum/Plasma/Urine Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF

PlGF (placenta growth factor) is a homodimeric growth factor that competes with VEGF for binding to VEGF R1/Flt-1. It therefore increases the availability of VEGF to bind to VEGF R2/KDR/Flk-1 and trigger angiogenesis. It can form heterodimers with some forms of VEGF and decrease the angiogenic effect of VEGF on VEGF R2. Circulating PlGF levels increase during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. PlGF induces monocyte activation and migration as well as production of inflammatory cytokines and VEGF. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. PlGF can also inhibit TIMP3 expression in the spleen, leading to immune triggering of hypertension.

Long Name:
Placenta Growth Factor
Entrez Gene IDs:
5228 (Human); 18654 (Mouse)
Alternate Names:
D12S1900; PGF; PGFL; placenta growth factor; placental growth factor; placental growth factor, vascular endothelial growth factor-related protein; PlGF; PlGF-2; PLGFplacental growth factor-like; SHGC-10760
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Serum, Plasma, & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human PlGF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

79 Citations: Showing 1 - 10
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  1. Angiogenic and Microvascular Status Alterations after Endovascular Revascularization of Lower Limb Arteries among Patients with Diabetic Foot Syndrome: A Prospective 12-Month Follow-Up Study
    Authors: Schönborn, M;Gregorczyk-Maga, I;Batko, K;Maga, M;Bogucka, K;Gawlik, K;Pawlica-Gosiewska, D;Maga, P;
    Journal of clinical medicine
    Species: Human
    Sample Types: Plasma
  2. Increased Interleukin-36 beta Expression Promotes Angiogenesis in Japanese Atopic Dermatitis
    Authors: Komaki, R;Miyagaki, T;Tanaka, M;Nakajima, K;Okano, T;Takeuchi, S;Kadono, T;
    International journal of molecular sciences
    Species:  Human
    Sample Types: Cell Culture Supernates, Serum
  3. Circulating Angiogenic Factors and Ischemic Diabetic Foot Syndrome Advancement-A Pilot Study
    Authors: Schönborn, M;Gregorczyk-Maga, I;Batko, K;Bogucka, K;Maga, M;P?otek, A;Pasieka, P;S?owi?ska-Solnica, K;Maga, P;
    Biomedicines
    Species: Human
    Sample Types: Plasma
  4. sFlt-1-enriched exosomes induced endothelial cell dysfunction and a preeclampsia-like phenotype in mice
    Authors: X Huang, L Jia, Y Jia, X Xu, R Wang, M Wei, H Li, H Peng, Y Wei, Q He, K Wang
    Cytokine, 2023-04-14;166(0):156190.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Serum
  5. Alterations in Antioxidant Micronutrient Concentrations in Placental Tissue, Maternal Blood and Urine and the Fetal Circulation in Pre-eclampsia
    Authors: LO Kurlak, PJ Scaife, LV Briggs, F Broughton, DS Gardner, HD Mistry
    International Journal of Molecular Sciences, 2023-02-10;24(4):.
    Species: Human
    Sample Types: Plasma
  6. Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/?-catenin signaling to alleviate preeclampsia
    Authors: Yang, Z;Jia, X;Deng, Q;Luo, M;Hou, Y;Yue, J;Mei, J;Shan, N;Wu, Z;
    International immunopharmacology
    Species: Human
    Sample Types: Serum
  7. Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy
    Authors: T Tsai, M Alwees, MA Asaad, J Theile, V Kakkassery, HB Dick, T Schultz, SC Joachim
    PLoS ONE, 2023-01-20;18(1):e0280488.
    Species: Human
    Sample Types: Vitreous Humor
  8. Similar Pro- and Antiangiogenic Profiles Close to Delivery in Different Clinical Presentations of Two Pregnancy Syndromes: Preeclampsia and Fetal Growth Restriction
    Authors: W Dymara-Kon, M Laskowska, E Grywalska, A Hymos, A B?a?ewicz, B Leszczy?sk
    International Journal of Molecular Sciences, 2023-01-04;24(2):.
    Species: Human
    Sample Types: Serum
  9. The Association of IL-17 and PlGF/sENG Ratio in Pre-Eclampsia and Adverse Pregnancy Outcomes
    Authors: D Darmochwal, A Chara
    International Journal of Environmental Research and Public Health, 2022-12-31;20(1):.
    Species: Human
    Sample Types: Serum
  10. Vitreous protein networks around ANG2 and VEGF in proliferative diabetic retinopathy and the differential effects of aflibercept versus bevacizumab pre-treatment
    Authors: I Klaassen, P Avery, RO Schlingema, DHW Steel
    Scientific Reports, 2022-12-06;12(1):21062.
    Species: Human
    Sample Types: Vitreous Humor
  11. Biomarkers for anti-vascular endothelial growth factor drugs
    Authors: S Kuriyama, T Yamada, A Matsuda, G Takahashi, T Iwai, K Takeda, K Ueda, T Miyasaka, Y Yokoyama, S Shinji, H Sonoda, R Ohta, K Yonaga, S Kanaka, H Yoshida
    Oncology Letters, 2022-11-07;24(6):463.
    Species: Human
    Sample Types: Plasma
  12. Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia
    Authors: EO Anto, DA Coall, EA Asiamah, OO Afriyie, O Addai-Mens, YA Wiafe, W Owiredu, C Obirikoran, ME Annani-Ako, NA Titiloye, E Adua, E Acheampong, EA Adu, S Opoku, AO Anto, A Tawiah, Y Wang, W Wang
    PLoS ONE, 2022-03-21;17(3):e0265717.
    Species: Human
    Sample Types: Plasma
  13. Impact of Chronic Kidney Disease on the Associations of Cardiovascular Biomarkers With Adverse Outcomes in Patients With Suspected or Known Coronary Artery Disease: The EXCEED-J Study
    Authors: H Wada, T Shinozaki, M Suzuki, S Sakagami, Y Ajiro, J Funada, M Matsuda, M Shimizu, T Takenaka, Y Morita, K Yonezawa, H Matsubara, Y Ono, T Nakamura, K Fujimoto, A Ninomiya, T Kato, T Unoki, D Takagi, K Wada, M Wada, M Iguchi, H Yamakage, T Kusakabe, A Yasoda, A Shimatsu, K Kotani, N Satoh-Asah, M Abe, M Akao, K Hasegawa, EXCEED?J S
    Journal of the American Heart Association, 2022-01-20;11(3):e023464.
    Species: Human
    Sample Types: Serum
  14. STOX1 deficiency is associated with renin-mediated gestational hypertension and placental defects
    Authors: JG Parchem, K Kanasaki, SB Lee, M Kanasaki, JL Yang, Y Xu, KM Earl, RA Keuls, VH Gattone, R Kalluri
    JCI Insight, 2021-01-25;0(0):.
    Species: Mouse
    Sample Types: Serum
  15. Potency of Tokishakuyakusan in treating preeclampsia: Drug repositioning method by in vitro screening of the Kampo library
    Authors: K Yagi, K Mimura, T Tomimatsu, T Matsuyama, Y Kawanishi, A Kakigano, H Nakamura, M Endo, T Kimura
    PLoS ONE, 2020-12-30;15(12):e0244684.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Circulating miRNAs Associated with Dysregulated Vascular and Trophoblast Function as Target-Based Diagnostic Biomarkers for Preeclampsia
    Authors: S Kim, M Park, JY Kim, T Kim, JY Hwang, KS Ha, MH Won, S Ryoo, YG Kwon, YM Kim
    Cells, 2020-08-31;9(9):.
    Species: Human
    Sample Types: Serum
  17. Sleep-disordered breathing does not impact maternal outcomes in women with hypertensive disorders of pregnancy
    Authors: DL Wilson, ME Howard, AM Fung, FJ O'Donoghue, M Barnes, M Lappas, SP Walker
    PLoS ONE, 2020-04-27;15(4):e0232287.
    Species: Human
    Sample Types: Serum
  18. Diagnostic accuracy of sFlt1/PlGF ratio as a marker for preeclampsia
    Authors: P Nikuei, M Rajaei, N Roozbeh, F Mohseni, F Poordarvis, M Azad, S Haidari
    BMC Pregnancy Childbirth, 2020-02-07;20(1):80.
    Species: Human
    Sample Types: Serum
  19. Distinct downstream signaling and the roles of VEGF and PlGF in high glucose-mediated injuries of human retinal endothelial cells in culture
    Authors: W Jiao, JF Ji, W Xu, W Bu, Y Zheng, A Ma, B Zhao, Q Fan
    Sci Rep, 2019-10-25;9(1):15339.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Sulphadoxine-pyrimethamine plus azithromycin may improve birth outcomes through impacts on inflammation and placental angiogenesis independent of malarial infection
    Authors: HW Unger, AP Hansa, C Buffet, W Hasang, A Teo, L Randall, M Ome-Kaius, S Karl, AA Anuan, JG Beeson, I Mueller, SJ Stock, SJ Rogerson
    Sci Rep, 2019-02-19;9(1):2260.
    Species: Human
    Sample Types:
  21. Value of Placental Growth Factor as a Predictor of Adverse Events During the Acute Phase of Acute Decompensated Heart Failure
    Authors: Y Nakada, R Kawakami, M Matsui, T Ueda, T Nakano, H Nakagawa, T Nishida, K Onoue, T Soeda, S Okayama, M Watanabe, H Okura, Y Saito
    Circ. J., 2018-12-26;0(0):.
    Species: Human
    Sample Types: Serum
  22. Anti-inflammatory cytokine and angiogenic factors levels in vitreous samples of diabetic retinopathy patients
    Authors: T Tsai, S Kuehn, N Tsiampalis, MK Vu, V Kakkassery, G Stute, HB Dick, SC Joachim
    PLoS ONE, 2018-03-27;13(3):e0194603.
    Species: Human
    Sample Types: Vitreous Humor
  23. Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation
    Authors: M Perrotta, A Lori, L Carnevale, S Fardella, G Cifelli, R Iacobucci, F Mastroiaco, D Iodice, F Pallante, M Storto, G Lembo, D Carnevale
    Cardiovasc. Res., 2018-03-01;114(3):456-467.
    Species: Mouse
    Sample Types: Tissue Culture Supernates
    Applications: ELISA Capture
  24. Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome
    Authors: KYB Lamm, ML Johnson, J Baker Phil, MB Muntiferin, JM James, HN Jones, RW Redline, A Rokas, LJ Muglia
    Elife, 2018-01-08;7(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Endothelial Dysfunction in Severe Preeclampsia is Mediated by Soluble Factors, Rather than Extracellular Vesicles
    Authors: M O'Brien, D Baczyk, JC Kingdom
    Sci Rep, 2017-07-19;7(1):5887.
    Species: Human
    Sample Types: Tissue Culture Supernates
  26. Household air pollution and angiogenic factors in pregnant Nigerian women: A randomized controlled ethanol cookstove intervention
    Authors: A Dutta, K Brito, G Khramstova, A Mueller, S Chinthala, D Alexander, D Adu, T Ibigbami, J Olamijulo, A Odetunde, K Adigun, L Pruitt, O Olopade, O Ojengbede, S Rana, CO Olopade
    Sci. Total Environ., 2017-05-29;599(0):2175-2181.
    Species: Human
    Sample Types: Plasma
  27. Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease
    Authors: X Li, QY Yao, HC Liu, QW Jin, BL Xu, SC Zhang, CT Tu
    J. Cell. Mol. Med., 2017-04-05;0(0):.
    Species: Human
    Sample Types: Serum
  28. Elevated expression of placental growth factor is associated with airway-wall vascular remodelling and thickening in smokers with asthma
    Authors: D Wu, T Lai, Y Yuan, M Chen, J Xia, W Li, G Pan, B Yuan, Q Lv, Y Li, D Li, B Wu
    Sci Rep, 2017-02-21;7(0):43017.
    Species: Human
    Sample Types: Serum
  29. Spectrum of Factors Triggering Endothelial Dysfunction in PIH
    Authors: VS Jammalamad, P Abraham
    J Clin Diagn Res, 2016-12-01;10(12):BC14-BC17.
    Species: Human
    Sample Types: Serum
  30. Predicting first trimester pregnancy outcome: derivation of a multiple marker test
    Authors: S Senapati, MD Sammel, SF Butts, P Takacs, K Chung, KT Barnhart
    Fertil. Steril., 2016-10-26;106(7):1725-1732.e3.
    Species: Human
    Sample Types: Serum
  31. Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia
    Authors: Ingrid C Weel
    PLoS ONE, 2016-06-17;11(6):e0157584.
    Species: Human
    Sample Types: Tissue Homogenates
  32. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
    Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
    J Am Heart Assoc, 2016-04-18;5(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Inhibition of placental growth factor in renal cell carcinoma.
    Authors: Bessho H, Wong B, Huang D, Siew E, Huang D, Tan J, Ong C, Tan S, Matsumoto K, Iwamura M, Teh B
    Anticancer Res, 2015-01-01;35(1):531-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis.
    Authors: Hou H, Cheng S, Chung K, Kuo M, Yeh C, Chang B, Lu H, Wang H, Yu C
    Autophagy, 2014-06-25;10(9):1509-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Reduction of maternal circulating endothelial progenitor cells in human pregnancies with intrauterine growth restriction.
    Authors: Calcaterra F, Taddeo A, Colombo E, Cappelletti M, Martinelli A, Calabrese S, Mavilio D, Cetin I, Della Bella S
    Placenta, 2014-04-26;35(7):431-6.
    Species: Human
    Sample Types: Plasma
  36. Persistent urinary podocyte loss following preeclampsia may reflect subclinical renal injury.
    Authors: White W, Garrett A, Craici I, Wagner S, Fitz-Gibbon P, Butters K, Brost B, Rose C, Grande J, Garovic V
    PLoS ONE, 2014-03-24;9(3):e92693.
    Species: Human
    Sample Types: Serum
  37. Integrating multiple 'omics' analyses identifies serological protein biomarkers for preeclampsia.
    Authors: Liu, Linda Y, Yang, Ting, Ji, Jun, Wen, Qiaojun, Morgan, Alexande, Jin, Bo, Chen, Gongxing, Lyell, Deirdre, Stevenson, David K, Ling, Xuefeng, Butte, Atul J
    BMC Med, 2013-11-06;11(0):236.
    Species: Human
    Sample Types: Serum
  38. Placental growth factor and soluble Fms-like tyrosine kinase 1 in diabetic pregnancy: A possible relation to distal villous immaturity.
    Authors: El-Tarhouny S, Almasry S, Elfayomy A, Baghdadi H, Habib F
    Histol Histopathol, 2013-08-13;29(2):259-72.
    Species: Human
    Sample Types: Serum
  39. Advanced oxidation protein products enhances soluble Fms-like tyrosine kinase 1 expression in trophoblasts: a possible link between oxidative stress and preeclampsia.
    Authors: Huang Q, Wang S, Zhang M, Huang L, Tian J, Yu Y, Wang Z, Zhong M
    Placenta, 2013-07-27;34(10):949-52.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis.
    Authors: Chaturvedi, Pallavi, Gilkes, Daniele, Wong, Carmen C, Luo, Weibo, Zhang, Huafeng, Wei, Hong, Takano, Naoharu, Schito, Luana, Levchenko, Andre, Semenza, Gregg L
    J Clin Invest, 2012-12-17;123(1):189-205.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Cytokine and angiogenic factors associated with efficacy and toxicity of pazopanib in advanced soft-tissue sarcoma: an EORTC-STBSG study.
    Br. J. Cancer, 2012-07-17;107(4):639-45.
    Species: Human
    Sample Types: Serum
  42. Biomarkers for antitumor activity of bevacizumab in gastric cancer models.
    Authors: Yamashita-Kashima Y, Fujimoto-Ouchi K, Yorozu K, Kurasawa M, Yanagisawa M, Yasuno H, Mori K
    BMC Cancer, 2012-01-25;12(0):37.
    Species: Human
    Sample Types: Tissue Homogenates
  43. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
    Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
    BMC Cancer, 2011-07-05;11(0):286.
    Species: Human
    Sample Types: Serum
  44. Phase I and pharmacokinetic study of sunitinib in pediatric patients with refractory solid tumors: a children's oncology group study.
    Authors: Dubois SG, Shusterman S, Ingle AM, Ahern CH, Reid JM, Wu B, Baruchel S, Glade-Bender J, Ivy P, Grier HE, Adamson PC, Blaney SM
    Clin. Cancer Res., 2011-06-20;17(15):5113-22.
    Species: Human
    Sample Types: Plasma
  45. Myeloid Biomarkers Associated with Glioblastoma Response to Anti-VEGF Therapy with Aflibercept.
    Authors: de Groot JF, Piao Y, Tran H, Gilbert M, Wu HK, Liu J, Bekele BN, Cloughesy T, Mehta M, Robins HI, Lassman A, Deangelis L, Camphausen K, Chen A, Yung W, Prados M, Wen PY, Heymach JV
    Clin. Cancer Res., 2011-06-01;17(14):4872-4881.
    Species: Human
    Sample Types: Plasma
  46. Pharmacodynamic and pharmacogenetic angiogenesis-related markers of first-line FOLFOXIRI plus bevacizumab schedule in metastatic colorectal cancer.
    Authors: Loupakis F, Cremolini C, Fioravanti A
    Br. J. Cancer, 2011-03-15;104(8):1262-9.
    Species: Human
    Sample Types: Plasma
  47. Placental Growth Factor: A Promising Diagnostic Biomarker for Tubal Ectopic Pregnancy.
    Authors: Horne AW, Shaw JL
    J. Clin. Endocrinol. Metab., 2010-11-03;0(0):.
    Species: Human
    Sample Types: Serum
  48. Placental growth factor mediates aldosterone-dependent vascular injury in mice.
    Authors: Jaffe IZ, Newfell BG, Aronovitz M
    J. Clin. Invest., 2010-11-01;120(11):3891-900.
    Species: Human
    Sample Types: Tissue Homogenates
  49. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
    Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
    Angiogenesis, 2010-10-16;13(4):337-47.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Effect of smoking on circulating angiogenic factors in high risk pregnancies.
    Authors: Jeyabalan A, Powers RW, Clifton RG, Van Dorsten P, Hauth JC, Klebanoff MA, Lindheimer MD, Sibai B, Landon M, Miodovnik M, Caritis S, Roberts JM, Kuller J, Cotroneo M, Kamon T, Mercer B, Ramsey R, Paul R, Rabello Y, McCart D, Mueller E, Goldenberg R, Copper R, Sorokin Y, Norman G, Millinder A, Christmas JT, McCoy S, Elder S, Elder N, Carter B, Pemberton V, Thurnau G, Meier A, Minton V, Meis P, Swain M, Moawad AH, Jones P, Iams JD, Meadows S, Brenner S, Collins B, Newman RB, Carter SG, Romero R, Sabo V, Thom E, Bain RP, MacPherson C, Johnson D, Fischer ML, McNellis D, Spong C, Catz C, Yaffe S
    PLoS ONE, 2010-10-12;5(10):e13270.
    Species: Human
    Sample Types: Serum
  51. Soluble fms-Like tyrosine kinase 1 (sFlt1), endoglin and placental growth factor (PlGF) in preeclampsia among high risk pregnancies.
    Authors: Powers RW, Jeyabalan A, Clifton RG
    PLoS ONE, 2010-10-11;5(10):e13263.
    Species: Human
    Sample Types: Serum
  52. Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis.
    Authors: Ha CT, Wu JA, Irmak S, Lisboa FA, Dizon AM, Warren JW, Ergun S, Dveksler GS
    Biol. Reprod., 2010-03-24;83(1):27-35.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Placenta growth factor in sickle cell disease: association with hemolysis and inflammation.
    Authors: Brittain JE, Hulkower B, Jones SK, Strayhorn D, De Castro L, Telen MJ, Orringer EP, Hinderliter A, Ataga KI
    Blood, 2009-12-29;115(10):2014-20.
    Species: Human
    Sample Types: Plasma
  54. An automated method for the determination of the sFlt-1/PIGF ratio in the assessment of preeclampsia.
    Authors: Verlohren S, Galindo A, Schlembach D, Zeisler H, Herraiz I, Moertl MG, Pape J, Dudenhausen JW, Denk B, Stepan H
    Am. J. Obstet. Gynecol., 2009-10-21;202(2):161.e1-161.e1.
    Species: Human
    Sample Types: Serum
  55. Potential role for plasma placental growth factor in predicting coronary heart disease risk in women.
    Authors: Cassidy A, Chiuve SE, Manson JE, Rexrode KM, Girman CJ, Rimm EB
    Arterioscler. Thromb. Vasc. Biol., 2008-10-16;29(1):134-9.
    Species: Human
    Sample Types: Plasma
  56. Endometrial NK cells are special immature cells that await pregnancy.
    Authors: Manaster I, Mizrahi S, Goldman-Wohl D, Sela HY, Stern-Ginossar N, Lankry D, Gruda R, Hurwitz A, Bdolah Y, Haimov-Kochman R, Yagel S, Mandelboim O
    J. Immunol., 2008-08-01;181(3):1869-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Antitumor activity and biomarker analysis of sunitinib in patients with bevacizumab-refractory metastatic renal cell carcinoma.
    Authors: Rini BI, Michaelson MD, Rosenberg JE, Bukowski RM, Sosman JA, Stadler WM, Hutson TE, Margolin K, Harmon CS, DePrimo SE, Kim ST, Chen I, George DJ
    J. Clin. Oncol., 2008-08-01;26(22):3743-8.
    Species: Human
    Sample Types: Plasma
  58. Plasma levels of soluble Tie2 and vascular endothelial growth factor distinguish critical limb ischemia from intermittent claudication in patients with peripheral arterial disease.
    Authors: Findley CM, Mitchell RG, Duscha BD, Annex BH, Kontos CD
    J. Am. Coll. Cardiol., 2008-07-29;52(5):387-93.
    Species: Human
    Sample Types: Plasma
  59. Cigarette smoke exposure and angiogenic factors in pregnancy and preeclampsia.
    Authors: Jeyabalan A, Powers RW, Durica AR, Harger GF, Roberts JM, Ness RB
    Am. J. Hypertens., 2008-06-19;21(8):943-7.
    Species: Human
    Sample Types: Plasma
  60. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008-05-27;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Regulation of vascular endothelial growth factor-A and its soluble receptor sFlt-1 by luteinizing hormone in vivo: implication for ovarian follicle angiogenesis.
    Authors: Gutman G, Barak V, Maslovitz S, Amit A, Lessing JB, Geva E
    Fertil. Steril., 2008-03-17;89(4):922-6.
    Species: Human
    Sample Types: Serum
  62. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
    Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
    J. Am. Coll. Cardiol., 2007-06-04;49(24):2341-9.
    Species: Human
    Sample Types: Bone Marrow Fluid
  63. Multiple biomarker use for detection of adverse events in patients presenting with symptoms suggestive of acute coronary syndrome.
    Authors: Apple FS, Pearce LA, Chung A, Ler R, Murakami MM
    Clin. Chem., 2007-03-23;53(5):874-81.
    Species: Human
    Sample Types: Plasma
  64. Angiogenic growth factors in maternal and fetal serum in pregnancies complicated by intrauterine growth restriction.
    Authors: Wallner W, Sengenberger R, Strick R, Strissel PL, Meurer B, Beckmann MW, Schlembach D
    Clin. Sci., 2007-01-01;112(1):51-7.
    Species: Human
    Sample Types: Serum
  65. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006-10-01;169(4):1167-82.
    Species: Human
    Sample Types: Tissue Homogenates
  66. Soluble endoglin and other circulating antiangiogenic factors in preeclampsia.
    Authors: Levine RJ, Lam C, Qian C, Yu KF, Maynard SE, Sachs BP, Sibai BM, Epstein FH, Romero R, Thadhani R, Karumanchi SA
    N. Engl. J. Med., 2006-09-07;355(10):992-1005.
    Species: Human
    Sample Types: Serum
  67. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
    Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
    Glia, 2006-06-01;53(8):845-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Cardiac expression of placental growth factor predicts the improvement of chronic phase left ventricular function in patients with acute myocardial infarction.
    Authors: Iwama H, Uemura S, Naya N, Imagawa K, Takemoto Y, Asai O, Onoue K, Okayama S, Somekawa S, Kida Y, Takeda Y, Nakatani K, Takaoka M, Kawata H, Horii M, Nakajima T, Doi N, Saito Y
    J. Am. Coll. Cardiol., 2006-03-27;47(8):1559-67.
    Species: Human
    Sample Types: Plasma
  69. Circulating angiogenic factors in preeclampsia, gestational proteinuria, and preeclampsia superimposed on chronic glomerulonephritis.
    Authors: Masuyama H, Suwaki N, Nakatsukasa H, Masumoto A, Tateishi Y, Hiramatrsu Y
    Am. J. Obstet. Gynecol., 2006-02-01;194(2):551-6.
    Species: Human
    Sample Types: Serum
  70. Relation of various plasma growth factor levels in patients with stable angina pectoris and total occlusion of a coronary artery to the degree of coronary collaterals.
    Authors: Briguori C, Testa U, Colombo A, Petrucci E, Condorelli G, Airoldi F, Peschle C, Condorelli G
    Am. J. Cardiol., 2005-12-20;97(4):472-6.
    Species: Human
    Sample Types: Serum
  71. Urinary angiogenic factors cluster hypertensive disorders and identify women with severe preeclampsia.
    Authors: Buhimschi CS, Norwitz ER, Funai E, Richman S, Guller S, Lockwood CJ, Buhimschi IA
    Am. J. Obstet. Gynecol., 2005-03-01;192(3):734-41.
    Species: Human
    Sample Types: Urine
  72. Urinary placental growth factor and risk of preeclampsia.
    Authors: Levine RJ, Thadhani R, Qian C, Lam C, Lim KH, Yu KF, Blink AL, Sachs BP, Epstein FH, Sibai BM, Sukhatme VP, Karumanchi SA
    JAMA, 2005-01-05;293(1):77-85.
    Species: Human
    Sample Types: Urine
  73. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
    Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
    Biochem. Biophys. Res. Commun., 2004-12-03;325(1):24-31.
    Species: Human
    Sample Types: Cell Lysates
  74. Circulating angiogenic factors and the risk of preeclampsia.
    Authors: Levine RJ, Maynard SE, Qian C, Lim KH, England LJ, Yu KF, Schisterman EF, Thadhani R, Sachs BP, Epstein FH, Sibai BM, Sukhatme VP, Karumanchi SA
    N. Engl. J. Med., 2004-02-05;350(7):672-83.
    Species: Human
    Sample Types: Serum
  75. Overexpression of placenta growth factor contributes to the pathogenesis of pulmonary emphysema.
    Authors: Tsao PN, Su YN, Li H, Huang PH, Chien CT, Lai YL, Lee CN, Chen CA, Cheng WF, Wei SC, Yu CJ, Hsieh FJ, Hsu SM
    Am. J. Respir. Crit. Care Med., 2003-11-25;169(4):505-11.
    Species: Human
    Sample Types: Serum
  76. Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.
    Authors: Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA
    J. Clin. Invest., 2003-03-01;111(5):649-58.
    Species: Human
    Sample Types: Serum
  77. A critical role of placental growth factor in the induction of inflammation and edema formation.
    Authors: Oura H, Bertoncini J, Velasco P, Brown LF, Carmeliet P, Detmar M
    Blood, 2002-09-05;101(2):560-7.
    Species: Human
    Sample Types: Cell Lysates
  78. A longitudinal study of biochemical variables in women at risk of preeclampsia.
    Authors: Chappell LC, 107299, Seed PT, Briley A, Kelly FJ, Hunt BJ, Charnock-Jones DS, Mallet AI, Poston L
    Optimization of differentiation protocols of dental tissues stem cells to pancreatic beta-cells, 2002-07-01;187(1):127-36.
  79. Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
    Authors: Zhou Y, 2019, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
    2002-04-01;160(4):1405-23.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

  1. Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?

    • Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. 

      Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.

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Human PlGF Quantikine ELISA Kit
By Vincent Giacalone on 05/20/2023
Sample Tested: Cell culture supernatant

this is a reliable ELISA kit for PLGF in a variety of sample types. unlike some of the other quantikine kits this protocol requires 100 uL of sample however.


Human PlGF Quantikine ELISA Kit
By Andres Ocampo on 10/11/2022
Sample Tested: Human fibroblast

Human PIGF was evaluated in HUVEC cells extracted from fetal membranes and exposed to pro-inflammatory factors (IL-1b and TNF-a). A significant increase in PIGIF production was obtained, compared to the untreated cells.


Human PlGF Quantikine ELISA Kit
By Martin Torres on 09/23/2021
Sample Tested: Human fetal membranes

Human PIGF levels were evaluated in fetal membrane explants extracted from women voluntarily subjected to the caesarian procedure. Membranes were seeded in transwell inserts for 7 days, and PIGF levels were quantified. Since PIGF is essential for fetal development and health, quantification obtained from this kit was critical to assess its levels. Overall, the kit is straightforward to follow, and the instructions are very clear. The picture shows the PIGF levels after maintaing the fetal membranes for 4 and 8 h, without treatment (control) and treated with TNF-a