Human PlGF Quantikine ELISA Kit

R&D Systems | Catalog # DPG00

R&D Systems
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Key Product Details

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)

Sensitivity

7 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
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Product Summary for Human PlGF Quantikine ELISA Kit

The Quantikine Human PlGF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human PlGF in cell culture supernates, serum, plasma, and urine. The immunoassay kit contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human PlGF

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 40.9 138 541 46.9 149 555
Standard Deviation 2.4 7.3 38.2 6.4 13.5 32.9
CV% 5.9 5.3 7.1 13.6 9.1 5.9

Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 54.3 175 658 55.0 184 724
Standard Deviation 3.8 6.3 36.9 6.5 20.3 78.9
CV% 7.0 3.6 5.6 11.8 11.0 10.9

Recovery for Human PlGF Quantikine ELISA Kit

The recovery of PlGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 97 82-107
Citrate Plasma (n=5) 99 93-120
EDTA Plasma (n=5) 102 92-117
Heparin Plasma (n=5) 94 81-113
Serum (n=5) 96 88-118
Urine (n=4) 97 85-112

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of PlGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human PlGF ELISA Linearity

Scientific Data Images for Human PlGF Quantikine ELISA Kit

Human PlGF ELISA Cell Culture Supernate Standard Curve

Human PlGF ELISA Cell Culture Supernate Standard Curve

Human PlGF ELISA Serum/Plasma/Urine Standard Curve

Human PlGF ELISA Serum/Plasma/Urine Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF

PlGF (placenta growth factor) is secreted primarily by villous trophoblasts and decidual cells, with circulating levels increasing during pregnancy but attenuated in preeclampsia. PlGF signals through VEGF R1/Flt‑1 and Neuropilins, while VEGF binds both VEGF R1 and R2 but signals mainly through VEGF R2. PlGF reduces binding of VEGF to VEGF R1 and increases VEGF/VEGF R2‑mediated angiogenesis. It induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing and also contribute to inflammation in active sickle cell disease and atherosclerosis.
 

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Gene Symbol

PGF

Additional PlGF Products

Product Documents for Human PlGF Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PlGF Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human PlGF Quantikine ELISA Kit

Customer Reviews for Human PlGF Quantikine ELISA Kit (3)

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  • Human PlGF Quantikine ELISA Kit
    Name: Vincent Giacalone
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 05/20/2023
    this is a reliable ELISA kit for PLGF in a variety of sample types. unlike some of the other quantikine kits this protocol requires 100 uL of sample however.
    Human PlGF Quantikine ELISA Kit DPG00
  • Human PlGF Quantikine ELISA Kit
    Name: Andres Ocampo
    Sample Tested: Human fibroblast
    Verified Customer | Posted 10/11/2022
    Human PIGF was evaluated in HUVEC cells extracted from fetal membranes and exposed to pro-inflammatory factors (IL-1b and TNF-a). A significant increase in PIGIF production was obtained, compared to the untreated cells.
    Human PlGF Quantikine ELISA Kit DPG00
  • Human PlGF Quantikine ELISA Kit
    Name: Martin Torres
    Sample Tested: Human fetal membranes
    Verified Customer | Posted 09/23/2021
    Human PIGF levels were evaluated in fetal membrane explants extracted from women voluntarily subjected to the caesarian procedure. Membranes were seeded in transwell inserts for 7 days, and PIGF levels were quantified. Since PIGF is essential for fetal development and health, quantification obtained from this kit was critical to assess its levels. Overall, the kit is straightforward to follow, and the instructions are very clear. The picture shows the PIGF levels after maintaing the fetal membranes for 4 and 8 h, without treatment (control) and treated with TNF-a
    Human PlGF Quantikine ELISA Kit DPG00

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Protocols

View specific protocols for Human PlGF Quantikine ELISA Kit (DPG00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well.
  7.   For Serum, Plasma, & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  8.   Aspirate and wash 4 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human PlGF Quantikine ELISA Kit

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  • Q: Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?

    A: Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.

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