PLK1 Antibody (S07-3E0)

Name: PLK1 Antibody (S07-3E0)
Brand: Novus Biologicals
SKU: NBP3-15079
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15079

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-3E0 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide of human PLK1 (Uniprot#: P53350)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLK1 Antibody (S07-3E0)

Western Blot: PLK1 Antibody (S07-3E0) [NBP3-15079]

Western Blot: PLK1 Antibody (S07-3E0) [NBP3-15079] - Western blot detection of PLK1 in Jurkat, C6 cell lysates using PLK1 Rabbit mAb (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.

Immunocytochemistry/Immunofluorescence: PLK1 Antibody (S07-3E0) [NBP3-15079] -

Immunocytochemistry/Immunofluorescence: PLK1 Antibody (S07-3E0) [NBP3-15079] - Immunocytochemistry of PLK1 (green) in Hela using NBP3-15079 at dilution 1/20, and DAPI (blue)

Applications for PLK1 Antibody (S07-3E0)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLK1

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Gene Symbol

PLK1

Additional PLK1 Products

Product Documents for PLK1 Antibody (S07-3E0)

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Certificate of Analysis

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Product Specific Notices for PLK1 Antibody (S07-3E0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Intracellular Kinases

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