PLK4 Antibody

Novus Biologicals | Catalog # NBP1-33402

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PLK4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PLK4. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

109 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLK4 Antibody

PLK4 Antibody

Western Blot: PLK4 Antibody [NBP1-33402] -

Western Blot: PLK4 Antibody [NBP1-33402] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK4 antibody (NBP1-33402) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PLK4 Antibody [NBP1-33402]

Immunocytochemistry/ Immunofluorescence: PLK4 Antibody [NBP1-33402]

Immunocytochemistry/Immunofluorescence: PLK4 Antibody [NBP1-33402] - Methanol-fixed HeLa, using PLK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
PLK4 Antibody

Western Blot: PLK4 Antibody [NBP1-33402] -

Untreated (-) and treated (+) U2OS whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK4 antibody (NBP1-33402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for PLK4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLK4

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle.

Long Name

Serine/threonine-protein kinase PLK4

Alternate Names

PLK-4, Polo-like kinase 4, SAK, STK18

Gene Symbol

PLK4

Additional PLK4 Products

Product Documents for PLK4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PLK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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