PLZF Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-80894
![Western Blot: PLZF Antibody [NBP1-80894]](https://beta-resources.rndsystems.com/images/products/PLZF-Antibody-Western-Blot-NBP1-80894-img0007.jpg "Western Blot: PLZF Antibody [NBP1-80894]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (98%), Rat (97%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, IHC-F
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PLZF Antibody - BSA Free (NBP1-80894) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PLZF Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PLZF Antibody - BSA Free
Western Blot: PLZF Antibody [NBP1-80894]
Western Blot: PLZF Antibody [NBP1-80894] - Analysis in human cell line HELA.![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]](https://beta-resources.rndsystems.com/images/products/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0014.jpg "Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]")
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Pancreas shows weak nuclear positivity in exocrine glandular cells.![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]](https://beta-resources.rndsystems.com/images/products/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0009.jpg "Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]")
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human testis shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]](https://beta-resources.rndsystems.com/images/products/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0012.jpg "Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]")
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Cerebral cortex shows moderate nuclear positivity in neuronal cells.![Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]](https://beta-resources.rndsystems.com/images/products/PLZF-Antibody-Immunohistochemistry-Paraffin-NBP1-80894-img0013.jpg "Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]")
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894]
Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Lung shows moderate nuclear positivity in macrophages.Applications for PLZF Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Frozen
Reported in scientific literature (PMID: 31145102).
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PLZF
Long Name
Promyelocytic Leukemia Zinc Finger
Alternate Names
ZBTB16, ZNF145
Entrez Gene IDs
7704 (Human)
Gene Symbol
ZBTB16
UniProt
Additional PLZF Products
Product Documents for PLZF Antibody - BSA Free
Product Specific Notices for PLZF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PLZF Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
