PMCA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12964

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Canine (100%), Chicken (95%), Porcine (100%), Rabbit (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PMCA1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PMCA1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PMCA1 Antibody - BSA Free

Western Blot: PMCA1 Antibody [NBP3-12964]

Western Blot: PMCA1 Antibody [NBP3-12964]

Western Blot: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein by western blot analysis. HeLa whole cell extracts and membrane extracts(the extracts were unboiled )(30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) at a dilution of 1:1000.
Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: PMCA1 protein stained by PMCA1 antibody (NBP3-12964) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PMCA1 Antibody - BSA Free

Western Blot: PMCA1 Antibody [NBP3-12964] -

Boiled and unboiled A549 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Applications for PMCA1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC-FR-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PMCA1

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Alternate Names

ATPase, Ca++ transporting, plasma membrane 1, EC 3.6.3, EC 3.6.3.8, plasma membrane calcium ATPase, Plasma membrane calcium ATPase isoform 1, plasma membrane calcium pump, Plasma membrane calcium pump isoform 1, plasma membrane calcium-ATPase, PMCA1kb, PMCA1plasma membrane calcium-transporting ATPase 1

Gene Symbol

ATP2B1

Additional PMCA1 Products

Product Documents for PMCA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PMCA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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