PMP70 Antibody (CL2524) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44465

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2524

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P28288, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PMP70 Antibody (CL2524) - Azide and BSA Free

PMP70 Antibody (CL2524) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Staining in HeLa cell line with Anti-PMP70 monoclonal antibody).
PMP70 Antibody (CL2524) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Staining in U251 cell line with Anti-PMP70 monoclonal antibody).
PMP70 Antibody (CL2524) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Staining in MCF7 cell line with Anti-PMP70 monoclonal antibody).
PMP70 Antibody (CL2524) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Staining in A431 cell line with Anti-PMP70 monoclonal antibody).
PMP70 Antibody (CL2524) - Azide and BSA Free Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Analysis in human liver and pancreas tissues using NBP3-44465 antibody. Corresponding PMP70 RNA-seq data are presented for the same tissues.
PMP70 Antibody (CL2524) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody [NBP3-44465]

Staining in U2OS cell line with Anti-PMP70 monoclonal antibody).
PMP70 Antibody (CL2524) - Azide and BSA Free Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
PMP70 Antibody (CL2524) - Azide and BSA Free Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Staining of human fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.
PMP70 Antibody (CL2524) - Azide and BSA Free Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
PMP70 Antibody (CL2524) - Azide and BSA Free Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.
PMP70 Antibody (CL2524) - Azide and BSA Free Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Staining of human rectum shows moderate granular cytoplasmic positivity in glandular cells.
PMP70 Antibody (CL2524) - Azide and BSA Free Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Immunohistochemistry-Paraffin: PMP70 Antibody [NBP3-44465]

Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.

Applications for PMP70 Antibody (CL2524) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PMP70

PMP70 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alternate Names

70 kDa peroxisomal membrane protein, ABC43, ATP-binding cassette, sub-family D (ALD), member 3, peroxisomal membrane protein 1 (70kD, Zellweger syndrome), Peroxisomal membrane protein-1 (70kD), PMP70ATP-binding cassette sub-family D member 3, PXMP1dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1)), ZWS2

Gene Symbol

ABCD3

Additional PMP70 Products

Product Documents for PMP70 Antibody (CL2524) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PMP70 Antibody (CL2524) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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