PNPT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-22269

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PNPT1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 700 and 750 of human Polynucleotide Phosphorylase 1 using the numbering given in entry NP_149100.2 (GeneID 87178).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PNPT1 Antibody - BSA Free

Western Blot: PNPT1 Antibody [NBP2-22269]

Western Blot: PNPT1 Antibody [NBP2-22269]

Western Blot: PNPT1 Antibody [NBP2-22269] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22269 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: PNPT1 Antibody [NBP2-22269]

Immunoprecipitation: PNPT1 Antibody [NBP2-22269]

Immunoprecipitation: PNPT1 Antibody [NBP2-22269] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22269 used for IP at 6 ug/mg lysate. PNPT1 was also immunoprecipitated by rabbit anti-PNPT1 antibody. For blotting immunoprecipitated PNPT1, NBP2-22269 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PNPT1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PNPT1

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

Alternate Names

DKFZp762K1914, EC 2.7.7, EC 2.7.7.8, mitochondrial, OLD35, old-35, PNPase, PNPase 1, PNPase old-35, Polynucleotide phosphorylase 1, Polynucleotide phosphorylase-like protein, polyribonucleotide nucleotidyltransferase 1

Gene Symbol

PNPT1

UniProt

NP_149100.2 (Human)

Additional PNPT1 Products

Product Documents for PNPT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PNPT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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