Podocalyxin Like Antibody (3D3) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-45167

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D3

Format

IHC-Prediluted
View all Podocalyxin Primary Antibodies »

Product Specifications

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27771514).

Localization

Cell surface and cytoplasmic

Marker

Hematopoietic Stem Cell Marker

Specificity

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Podocalyxin Like Antibody (3D3) - IHC-Prediluted

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Like Antibody (3D3).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (3D3).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Like Antibody (3D3).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).
Podocalyxin Like Antibody (3D3) - IHC-Prediluted Western Blot: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] -

Western Blot: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] -

Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3) - IHC-Prediluted.
Podocalyxin Like Antibody (3D3) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] -

Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] -

Confocal Immunofluorescence of HeLa cells using Podocalyxin Like Antibody (3D3) - IHC-Prediluted labeled with CF488 (Green); Reddot is used to label the nuclei.
Podocalyxin Like Antibody (3D3) - IHC-Prediluted Flow Cytometry: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] -

Flow Cytometry: Podocalyxin Like Antibody (3D3) - IHC-Prediluted [NBP2-45167] -

Flow Cytometry of NCCIT cells using Podocalyxin Like Antibody (3D3) - IHC-Prediluted.

Applications for Podocalyxin Like Antibody (3D3) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (3D3) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for Podocalyxin Like Antibody (3D3) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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