Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33108

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D3

Format

Azide and BSA Free
View all Podocalyxin Primary Antibodies »

Product Specifications

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27771514).

Localization

Cell surface and Cytoplasmic

Marker

Hematopoietic Stem Cell Marker

Specificity

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide.

Antibody without azide - store at -20 to -80C.

Scientific Data Images for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Western Blot: Podocalyxin Like Antibody (3D3)Azide and BSA Free [NBP2-33108]

Western Blot: Podocalyxin Like Antibody (3D3)Azide and BSA Free [NBP2-33108]

Western Blot: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3).
Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Confocal Immunofluorescence of HeLa cells using Podocalyxin Like Antibody (3D3) labeled with CF488 (Green); Red Dot is used to label the nuclei.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).
Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108PE and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the Azide and BSA Free form of this antibody.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (3D3).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Like Antibody (3D3).
Flow Cytometry: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Flow Cytometry: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Flow Cytometry: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Surface staining of NCCIT cells using Podocalyxin antibody at 1 ug/10^6 cells. A FITC-conjugated anti mouse Ig secondary antibody was used along with this antibody.
Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Flow (Intracellular): Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - An intracellular stain was performed on HeLa cells with Podocalyxin Like Antibody (3D3) NBP2-33108APC and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.

Applications for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Immunoprecipitation

0.5-1ug/500ug protein lysate

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

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Protocols

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FAQs

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