Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 56F7

Format

BSA Free
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Podoplanin (99-207aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse Podoplanin Antibody (56F7) - BSA Free (NBP2-59445) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Podoplanin Antibody (56F7) - BSA Free

Western Blot: Podoplanin Antibody (56F7)BSA Free [NBP2-59445]

Western Blot: Podoplanin Antibody (56F7)BSA Free [NBP2-59445]

Western Blot: Podoplanin Antibody (56F7) [NBP2-59445] - Analysis in HeLa cell lysates (40ug).
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (56F7) - BSA Free [NBP2-59445]

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (56F7) - BSA Free [NBP2-59445]

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (56F7) [NBP2-59445] - Analysis of PDPN in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDPN antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Podoplanin Antibody (56F7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (56F7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Podoplanin Antibody (56F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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