Podoplanin Antibody (NZ-1.2) - BSA Free

Novus Biologicals | Catalog # NBP2-03952

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Lambda Clone # NZ-1.2

Format

BSA Free
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 38-70 from the platelet-aggregation-stimulating (PLAG) domain of human podoplanin was used as immunogen.

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Lambda

Scientific Data Images for Podoplanin Antibody (NZ-1.2) - BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.
Western Blot: Podoplanin Antibody (NZ-1.2)BSA Free [NBP2-03952]

Western Blot: Podoplanin Antibody (NZ-1.2)BSA Free [NBP2-03952]

Western Blot: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Detection of lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/ml.
Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - A surface stain was performed on Ntera2 cells with Podoplanin Antibody (NZ-1.2) NBP2-03952AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).
Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).
Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 0.2 ug of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green). Flow kit and anti-rat PE secondary were used for this test.
Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) - BSA Free [NBP2-03952]

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the PE conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.2 ug) of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green).

Applications for Podoplanin Antibody (NZ-1.2) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.2-1 ug/10^6 cells

Immunohistochemistry-Frozen

0.5-1ug/ml. Use reported in scientific literature (Kaji et al (2012); PMID 23012488)

Immunohistochemistry-Paraffin

0.5-1ug / ml. Use reported in scientific literature (Kaji et al (2012); PMID 23012488)

Western Blot

3-5 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is a mucin type-1 glycoprotein of 40-43kd also know as T1A, gp38 or Aggrus. Podoplanin is expressed in many tumors and many normal cells, especially lymphatic epithelial cells and follicular DCs. It appears to serve as a ligand for CLEC-2. Podoplanin expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue (Schacht, 2005, Raica, 2008, Martin-Villar, 2010). It has also been determined that podoplanin expression is induced through Src activation of Cas (Shen, 2010). Recently there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (NZ-1.2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Podoplanin Antibody (NZ-1.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Podoplanin Antibody (NZ-1.2) - BSA Free

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Protocols

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