POGZ Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83005

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POGZ Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GEPWCDVVLAILADGTVLPTLVFYRGQMDQPANMPDSILLEAKESGYSDDEIMELWSTRVWQKHTACQRSKGMLVMDCHRTHLSEEVLAMLSASSTLPAVVPAGCSSKIQPLDVCIKRTVKNFLH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POGZ Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: POGZ Antibody [NBP1-83005]

Immunocytochemistry/ Immunofluorescence: POGZ Antibody [NBP1-83005]

Immunocytochemistry/Immunofluorescence: POGZ Antibody [NBP1-83005] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-POGZ antibody NBP1-83005 (A) shows similar protein distribution across tissues to independent antibody NBP1-83004 (B).
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human testis.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human lymph node.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human colon.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005]

Immunohistochemistry-Paraffin: POGZ Antibody [NBP1-83005] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Applications for POGZ Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POGZ

Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors. Mouse Pogz has a human homolog with a base pair similarity of 90% and amino acid similarity of 93% for the full length protein. In humans at least 3 variants have been predicted and they are all similar at the Nterminus. A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells. In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.

Alternate Names

KIAA0461ZNF635, pogo transposable element with ZNF domain, putative protein product of Nbla00003, SUHW5MGC71543, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635, ZNF280EZNF635m

Gene Symbol

POGZ

Additional POGZ Products

Product Documents for POGZ Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for POGZ Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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