POLR2D Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93656

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1). MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2D Antibody - BSA Free

Western Blot: POLR2D AntibodyBSA Free [NBP2-93656]

Western Blot: POLR2D AntibodyBSA Free [NBP2-93656]

Western Blot: POLR2D Antibody [NBP2-93656] - Analysis of 200ug extracts of HepG2 cells using POLR2D at a dilition of 1:1000.
Immunocytochemistry/ Immunofluorescence: POLR2D Antibody - BSA Free [NBP2-93656]

Immunocytochemistry/ Immunofluorescence: POLR2D Antibody - BSA Free [NBP2-93656]

Immunocytochemistry/Immunofluorescence: POLR2D Antibody [NBP2-93656] - Analysis of A549 cells using POLR2D.
Western Blot: POLR2D AntibodyBSA Free [NBP2-93656]

Western Blot: POLR2D AntibodyBSA Free [NBP2-93656]

Western Blot: POLR2D Antibody [NBP2-93656] - Analysis of extracts of various cell lines, using POLR2D at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications for POLR2D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunoprecipitation

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR2D

The POLR2D gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have

Alternate Names

DNA-directed RNA polymerase II 16 kDa polypeptide, DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, polymerase (RNA) II (DNA directed) polypeptide D, RBP4, RNA polymerase II 16 kDa subunit, RNA polymerase II subunit B4, RNA polymerase II subunit hsRBP4, RPB16

Gene Symbol

POLR2D

Additional POLR2D Products

Product Documents for POLR2D Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for POLR2D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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