Polypeptide GalNac Transferase 3/GALNT3 Antibody

Novus Biologicals | Catalog # NBP3-13034

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (97%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Polypeptide GalNAc Transferase 3/GALNT3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Polypeptide GalNac Transferase 3/GALNT3. The exact sequence is proprietary.

Reactivity Notes

Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Polypeptide GalNac Transferase 3/GALNT3 Antibody

Immunocytochemistry/ Immunofluorescence: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034]

Immunocytochemistry/ Immunofluorescence: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034]

Immunocytochemistry/Immunofluorescence: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] - Polypeptide GalNac Transferase 3/GALNT3 antibody detects Polypeptide GalNac Transferase 3/GALNT3 protein at Golgi apparatus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Polypeptide GalNac Transferase 3/GALNT3 stained by Polypeptide GalNac Transferase 3/GALNT3 antibody (NBP3-13034) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Polypeptide GalNac Transferase 3/GALNT3 Antibody

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] -

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] - Polypeptide GalNac Transferase 3/GALNT3 antibody detects Polypeptide GalNac Transferase 3/GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Polypeptide GalNac Transferase 3/GALNT3 stained by Polypeptide GalNac Transferase 3/GALNT3 antibody (NBP3-13034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Polypeptide GalNac Transferase 3/GALNT3 Antibody

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] -

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] - Polypeptide GalNac Transferase 3/GALNT3 antibody detects Polypeptide GalNac Transferase 3/GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Polypeptide GalNac Transferase 3/GALNT3 stained by Polypeptide GalNac Transferase 3/GALNT3 antibody (NBP3-13034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Polypeptide GalNac Transferase 3/GALNT3 Antibody

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] -

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] - Polypeptide GalNac Transferase 3/GALNT3 antibody detects Polypeptide GalNac Transferase 3/GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Polypeptide GalNac Transferase 3/GALNT3 stained by Polypeptide GalNac Transferase 3/GALNT3 antibody (NBP3-13034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Polypeptide GalNac Transferase 3/GALNT3 Antibody

Immunocytochemistry/ Immunofluorescence: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] -

Immunocytochemistry/ Immunofluorescence: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] - Polypeptide GalNac Transferase 3/GALNT3 antibody detects Polypeptide GalNac Transferase 3/GALNT3 protein at Golgi by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Polypeptide GalNac Transferase 3/GALNT3 stained by Polypeptide GalNac Transferase 3/GALNT3 antibody (NBP3-13034) diluted at 1:3000.
Blue: Fluoroshield with DAPI.
Polypeptide GalNac Transferase 3/GALNT3 Antibody

Western Blot: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] -

Western Blot: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Polypeptide GalNac Transferase 3/GALNT3 antibody (NBP3-13034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Polypeptide GalNac Transferase 3/GALNT3 Antibody

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] -

Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 3/GALNT3 Antibody [NBP3-13034] - Polypeptide GalNac Transferase 3/GALNT3 antibody detects Polypeptide GalNac Transferase 3/GALNT3 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Polypeptide GalNac Transferase 3/GALNT3 stained by Polypeptide GalNac Transferase 3/GALNT3 antibody (NBP3-13034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Polypeptide GalNac Transferase 3/GALNT3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Polypeptide GalNAc Transferase 3/GALNT3

GALNT3, also known as Polypeptide N-acetylgalactosaminyltransferase 3, has a 633 amino acid long isoform that is 73 kDa and a short 192 amino acid isoform that is 22 kDa; is located in the Golgi apparatus; plays a central role in phosphate homeostasis; acts as a catalyzer in the initiation of O-linked oligosaccharide biosynthesis, and the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor; shows an activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5; and glycosylates FGF23, and probably fibronectin in vivo too. Studies on this protein have shown a relationship with the following diseases and disorders: tumoral calcinosis, hyperphosphatemic calcinosis, hyperostosis-hyperphosphatemia syndrome, hyperphosphatemic familial tunoral calcinisis, normophosphatemic familial tumoral calcinosis, testicular microlithiasis, bile duct carcinoma, periostitis, squamous cell carcinoma, diabetes mellitus, colon adenocarcinoma, lung adenocarcinoma, pancreatic cancer, metabolic disorders, and lung cancer. This protein has also been shown to have interactions with CIGALT1, CIGALT1C1, ST6GALNAC1, B3GNT6, and GCNT1 in pathways such as O-linked glycosylation of mucins, metabolism of proteins, and post-translational protein modification.

Long Name

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3

Alternate Names

GalNAc-T3, HFTC, HHS, pp-GaNTase 3

Gene Symbol

GALNT3

Additional Polypeptide GalNAc Transferase 3/GALNT3 Products

Product Documents for Polypeptide GalNac Transferase 3/GALNT3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Polypeptide GalNac Transferase 3/GALNT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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Protocols

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