IL-12 and IL-23 are disulfide-linked heterodimers consisting of a p40 subunit that is common to both cytokines and a unique subunit, p19 in IL-23 or p35 in IL-12.
Porcine IL-12/IL-23 p40 Antibody (116211) [Biotin]
R&D Systems | Catalog # BAM9122
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Porcine
Cited:
Porcine
Applications
Validated:
ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Development (Detection)
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2B Clone # 116211
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p70 heterodimer
Specificity
Detects the p40 subunit of porcine IL-12 and IL-23. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human (rh) IL‑12 and rhIL‑23 and no cross-reactivity with rmIL-12 or rmIL-23.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Porcine IL-12/IL-23 p40 Antibody (116211) [Biotin]
Porcine IL-12 / IL-23 p40 ELISA Standard Curve
Recombinant Porcine IL‑12 (Catalog # 912-PL) was serially diluted and captured by Mouse Anti-Porcine IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB9121) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine IL‑12/IL‑23 p40 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Porcine IL-12/IL-23 p40 Antibody (116211) [Biotin]
Porcine IL-12 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-12/IL-23 p40
Long Name
Interleukin 12/Interleukin 23 p40
Alternate Names
CLMF2, IL-12B, IL12 p40, IL12B, NKSF2
Entrez Gene IDs
Gene Symbol
IL12B
Additional IL-12/IL-23 p40 Products
Product Documents for Porcine IL-12/IL-23 p40 Antibody (116211) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Porcine IL-12/IL-23 p40 Antibody (116211) [Biotin]
For research use only
Citations for Porcine IL-12/IL-23 p40 Antibody (116211) [Biotin]
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