Porcine IL-8/CXCL8 Antibody

(2 citations)   
  • Species Reactivity
    Porcine
  • Specificity
    Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL8 or rhCXCL1, 2, 3, 5, 6, 7, 9, 10, 11, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12, 13, recombinant rat CXCL1, 2 or 3 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 105115
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant porcine IL-8/CXCL8
    Ala26-Gln104
    Accession # CAA43461
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN)
  • Neutralization
    Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by
IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody.

Recombinant Porcine
IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine
IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine CXCL8/IL‑8 Monoclonal Antibody (Catalog # MAB535). The ND50 is typically
0.2‑1.0 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8

CXCL8, also called Interleukin 8 (IL-8), neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI), is a neutrophil chemotactic and activating factor in the CXC chemokine family. Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes, an autocrine growth factor for melanoma cells, and an angiogenic chemokine.

  • References:
    1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
  • Long Name:
    Interleukin 8
  • Entrez Gene IDs:
    3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
  • Alternate Names:
    3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant
    Authors: C Martin, SD Waghela, S Lokhandwal, A Ambrus, J Bray, C Vuong, V Vinodkumar, PJ Dominowski, S Rai, D Mwangi, DL Foss, W Mwangi
    PLoS ONE, 2017;12(1):e0170504.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Flow
  2. Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells.
    Authors: Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP
    J. Gen. Virol., 2004;85(0):1029-37.
    Species: Porcine
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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