Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL8 or rhCXCL1, 2, 3, 5, 6, 7, 9, 10,
11, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12, 13, recombinant rat
CXCL1, 2 or 3 is observed.
Monoclonal Mouse IgG1 Clone # 105115
Protein A or G purified from ascites
E. coli-derived recombinant porcine IL-8/CXCL8 Ala26-Gln104 Accession # CAA43461
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody.
Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine CXCL8/IL‑8 Monoclonal Antibody (Catalog # MAB535). The ND50 is typically 0.2‑1.0 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL8, also called Interleukin 8 (IL-8), neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI), is a neutrophil chemotactic and activating factor in the CXC chemokine family. Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes, an autocrine growth factor for melanoma cells, and an angiogenic chemokine.
Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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