CXCL8, also called Interleukin 8 (IL-8), neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI), is a neutrophil chemotactic and activating factor in the CXC chemokine family. Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes, an autocrine growth factor for melanoma cells, and an angiogenic chemokine.
Discontinued Product
MAB535 has been discontinued.
An alternative/replacement product is available:
MAB5351. View all IL-8/CXCL8 products.
Key Product Details
Species Reactivity
Validated:
Porcine
Cited:
Porcine
Applications
Validated:
Western Blot, Neutralization
Cited:
Flow Cytometry, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 105115
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Product Specifications
Immunogen
E. coli-derived recombinant porcine IL-8/CXCL8
Ala26-Gln104
Accession # CAA43461
Ala26-Gln104
Accession # CAA43461
Specificity
Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL8 or rhCXCL1, 2, 3, 5, 6, 7, 9, 10,
11, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12, 13, recombinant rat
CXCL1, 2 or 3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Porcine IL-8/CXCL8 Antibody
Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody.
Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine CXCL8/IL-8 Monoclonal Antibody (Catalog # MAB535). The ND50 is typically 0.2-1.0 µg/mL.Applications for Porcine IL-8/CXCL8 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN)
Sample: Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN)
Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8.
Reviewed Applications
Read 1 review rated 5 using MAB535 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-8/CXCL8
References
- Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
Long Name
Interleukin 8
Alternate Names
CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1
Gene Symbol
CXCL8
UniProt
Additional IL-8/CXCL8 Products
Product Documents for Porcine IL-8/CXCL8 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Porcine IL-8/CXCL8 Antibody
For research use only
Citations for Porcine IL-8/CXCL8 Antibody
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Application: MSD assaySample Tested: Vitreous humorSpecies: PorcineVerified Customer | Posted 10/18/2018After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC. Paired with goat PC IL-8 antibody (AF535) as a detection antibody. A standard curve with recombinant porcine IL-8 from RnD Cat# 535-IN/CF is shown 2-25,000 pg/ml.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
