POU4F3 Antibody
Novus Biologicals | Catalog # NBP1-52036
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-EAAYREKNSKPE corresponding to internal region according to NP_002691.1.
Epitope
C-EAAYREKNSKPE
Specificity
This antibody is expected not to cross-react to the similar POU4F1 and POU4F2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for POU4F3 Antibody
Western Blot: POU4F3 Antibody [NBP1-52036]
Western Blot: POU4F3 Antibody [NBP1-52036] - Analysis of POU4F3 in Human Heart lysate (35ug protein in RIPA buffer) at 0.2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for POU4F3 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in human and mouse heart lysates (calculated MW of 37.1 kDa band according to human NP_002691.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: POU4F3
Alternate Names
Brain-3C, Brain-specific homeobox/POU domain protein 3C, BRN3.1, Brn-3C, BRN3Cbrn-3C, DFNA15, MGC138412, POU class 4 homeobox 3, POU domain class 4, transcription factor 3, POU domain, class 4, transcription factor 3
Entrez Gene IDs
5459 (Human)
Gene Symbol
POU4F3
UniProt
Additional POU4F3 Products
Product Documents for POU4F3 Antibody
Certificate of Analysis
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Product Specific Notices for POU4F3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for POU4F3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
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- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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