POU6F2 Antibody

Novus Biologicals | Catalog # NBP1-52038

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all POU6F2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence RSEMNAELRGEDK-C corresponding to N-Terminus according to NP_009183.3, NP_001159490.1.

Epitope

RSEMNAELRGEDK-C

Specificity

This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for POU6F2 Antibody

Western Blot: POU6F2 Antibody [NBP1-52038]

Western Blot: POU6F2 Antibody [NBP1-52038]

Western Blot: POU6F2 Antibody [NBP1-52038] - NBP1-52038 (0.3ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for POU6F2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in mouse eye lysates (calculated MW of 73.3 kDa band according to human NP_009183.3 and mouse NP_778171.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POU6F2

POU6F2 is a member of a gene family characterized by the presence of a bipartite DNA-binding domain, consisting of a POU-specific domain and a POU heterodomain, separated by a variable polylinker. POU domain family members are transcriptional regulators, many of which show highly restricted patterns of expression and are known to control cell type-specific differentiation pathways (see review by Phillips and Luisi, 2000 [PubMed 11183772]).[supplied by OMIM]

Alternate Names

POU class 6 homeobox 2, Retina-derived POU domain factor 1, retina-derived POU-domain factor-1, RPF1, RPF-1, RPF-1class 6, transcription factor 2, Wilms tumor suppressor locus, WT5, WTSL

Entrez Gene IDs

11281 (Human)

Gene Symbol

POU6F2

UniProt

NP_009183.3 (Human)

Additional POU6F2 Products

Product Documents for POU6F2 Antibody

Certificate of Analysis

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Product Specific Notices for POU6F2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POU6F2 Antibody

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