PP2A alpha [p Tyr307] Antibody (23GB4580)

Novus Biologicals | Catalog # NBP3-50359

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4580
View all PP2A Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-PP2A alpha (Y307) (Uniprot: P67775)

Modification

p Tyr307

Specificity

This antibody detects endogenous levels of PPP2CA only when phosphorylated at Y307

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2A alpha [p Tyr307] Antibody (23GB4580)

PP2A alpha [p Tyr307] Antibody (23GB4580)

Immunocytochemistry/ Immunofluorescence: PP2A alpha [p Tyr307] Antibody (23GB4580)[NBP3-50359] -

Immunocytochemical staining of HAP-1 cells with PP2A alpha (1:1,000). Nuclei were stained blue with DAPI; Phospho-PP2A alpha (Y307)  was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.

Applications for PP2A alpha [p Tyr307] Antibody (23GB4580)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PP2A

Protein phosphatase type 2A (PP2A ) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways. PP2A is comprised of a core enzyme (which is made up of catalytic C and regulatory A (PR65) subunits) and a secondary regulatory B subunit. PP2A alpha refers to the alpha isoform of the PP2A catalytic subunit.

Alternate Names

PP2CA, PPP2CA, RP-C

Gene Symbol

PPP2CA

Additional PP2A Products

Product Documents for PP2A alpha [p Tyr307] Antibody (23GB4580)

Certificate of Analysis

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Product Specific Notices for PP2A alpha [p Tyr307] Antibody (23GB4580)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail