PP2C alpha/PPM1A Antibody (7F12) - BSA Free

Novus Biologicals | Catalog # NBP2-37480

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7F12

Format

BSA Free
View all PP2C alpha/PPM1A Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PP2C alpha/PPM1A (AA: 202-382) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PP2C alpha/PPM1A Antibody (7F12) - BSA Free

Western Blot: PP2C alpha/PPM1A Antibody (7F12)BSA Free [NBP2-37480]

Western Blot: PP2C alpha/PPM1A Antibody (7F12)BSA Free [NBP2-37480]

Western Blot: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Western blot analysis using PPM1A mouse mAb against Jurkat (1), Jurkat (2), A431 (3), HeLa (4), HEK293 (5), Raji (6), MCF-7 (7), and COS7 (8) cell lysate.
Flow Cytometry: PP2C alpha/PPM1A Antibody (7F12) - BSA Free [NBP2-37480]

Flow Cytometry: PP2C alpha/PPM1A Antibody (7F12) - BSA Free [NBP2-37480]

Flow Cytometry: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Flow cytometric analysis of HeLa cells using PPM1A mouse mAb (green) and negative control (red).
Western Blot: PP2C alpha/PPM1A Antibody (7F12)BSA Free [NBP2-37480]

Western Blot: PP2C alpha/PPM1A Antibody (7F12)BSA Free [NBP2-37480]

Western Blot: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Western blot analysis using PPM1A mAb against human PPM1A recombinant protein. (Expected MW is 45.9 kDa)
Western Blot: PP2C alpha/PPM1A Antibody (7F12)BSA Free [NBP2-37480]

Western Blot: PP2C alpha/PPM1A Antibody (7F12)BSA Free [NBP2-37480]

Western Blot: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Western blot analysis using PPM1A mAb against HEK293 (1) and PPM1A (AA: 202-382)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: PP2C alpha/PPM1A Antibody (7F12) - BSA Free [NBP2-37480]

ELISA: PP2C alpha/PPM1A Antibody (7F12) - BSA Free [NBP2-37480]

ELISA: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PP2C alpha/PPM1A Antibody (7F12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C alpha/PPM1A

Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C

Long Name

Protein Phosphatase 2C alpha

Alternate Names

MMPa-2, MPPa-1, Pp2c1, PPM1A

Gene Symbol

PPM1A

Additional PP2C alpha/PPM1A Products

Product Documents for PP2C alpha/PPM1A Antibody (7F12) - BSA Free

Certificate of Analysis

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Product Specific Notices for PP2C alpha/PPM1A Antibody (7F12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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