PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73548

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3C9

Format

Azide and BSA Free
View all PP2C beta/PPM1B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PPM1B (NP_002697) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free

Western Blot: PP2C beta/PPM1B Antibody (OTI3C9)Azide and BSA Free [NBP2-73548]

Western Blot: PP2C beta/PPM1B Antibody (OTI3C9)Azide and BSA Free [NBP2-73548]

Western Blot: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B.
Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Immunocytochemistry/Immunofluorescence: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PPM1B.
Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548] - Analysis of HEK293T cells transfected with either RC212918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by PPM1B antibody, and then analyzed by flow cytometry.
Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548] - Analysis of Hela cells, using PPM1B antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free [NBP2-73548] - Analysis of Jurkat cells, using PPM1B antibody,(Red), compared to a nonspecific negative control antibody (Blue).

Applications for PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2C beta/PPM1B

PPM1B is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.

Long Name

Protein Phosphatase 2C beta

Alternate Names

Pp2c2, PP2CB, PPM1B

Gene Symbol

PPM1B

Additional PP2C beta/PPM1B Products

Product Documents for PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PP2C beta/PPM1B Antibody (OTI3C9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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