PPP2R1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19907

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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPP2R1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (88%), Xenopus laevis (95%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPP2R1A Antibody - BSA Free

Western Blot: PPP2R1A Antibody [NBP2-19907]

Western Blot: PPP2R1A Antibody [NBP2-19907]

Western Blot: PPP2R1A Antibody [NBP2-19907] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:5000.
PPP2R1A Antibody

Immunohistochemistry-Paraffin: PPP2R1A Antibody [NBP2-19907] -

Immunohistochemistry-Paraffin: PPP2R1A Antibody [NBP2-19907] - PPP2R1A antibody detects PPP2R1A protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PPP2R1A antibody (NBP2-19907) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PPP2R1A Antibody - BSA Free

Western Blot: PPP2R1A Antibody [NBP2-19907] -

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP2R1A antibody (NBP2-19907) diluted at 1:1000.
PPP2R1A Antibody - BSA Free

Western Blot: PPP2R1A Antibody - BSA Free [NBP2-19907] -

Protein expression levels of PPP2R5D and its interaction with the PPP2CA and PPP2R1A. (A) HA-tagged PPP2R5D WT/variants were transfected in 293T cells, protein expression was detected through Western blot. A 66 kDa band was detected in all missense variants. As for frameshift variant H436Mfs*3, a ~50 kDa band was detected and it was significantly weaker. (B) HA-tagged PPP2R5D WT/variants were purified through HA pull down, the interaction with endogenous PPP2CA and PPP2R1A were detected through Western blot. No band of PPP2CA and PPP2R1A was detected in W207R, D251H, D251Y and H463Mfs*3. A weaker band of PPP2CA and PPP2R1A was detected in E198K, E200K, L203P and Q211P. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2227-9067/11/8/897), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PPP2R1A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:2500

Immunohistochemistry-Paraffin

1:100-1:2500

Western Blot

1:500-1:10000
Application Notes
Immunoprecipitation Assay-dependent dilution

Reviewed Applications

Read 1 review rated 4 using NBP2-19907 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPP2R1A

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq]

Alternate Names

Medium tumor antigen-associated 61 kDa protein, MGC786, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PP2AAALPHA, PP2A-Aalpha, PR65A, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, protein phosphatase 2, regulatory subunit A, alpha, serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform

Gene Symbol

PPP2R1A

UniProt

P30153 (Human)

Additional PPP2R1A Products

Product Documents for PPP2R1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PPP2R1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PPP2R1A Antibody - BSA Free

Customer Reviews for PPP2R1A Antibody - BSA Free (1)

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  • PPP2R1A Antibody
    Name: Agbolahan Sofela
    Application: Western Blot
    Sample Tested: Cultured cancer cell lysates and Human Whole Cell lysate
    Species: Human
    Verified Customer | Posted 09/30/2019
    These have been attached. The protein content of all samples were quantified prior to loading, and standardized at concentrations of 40ug.
    PPP2R1A Antibody - BSA Free NBP2-19907

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