PRAGMIN Antibody
Novus Biologicals | Catalog # NBP1-42689
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 275 and 325 of human homolog of rat pragma of Rnd2 using the numbering given in entry NP_001074295.1 (GeneID 157285).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PRAGMIN Antibody
Western Blot: PRAGMIN Antibody [NBP1-42689]
Western Blot: PRAGMIN Antibody [NBP1-42689] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pragmin antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Pragmin was also immunoprecipitated by another rabbit anti-Pragmin antibody, which recognizes a downstream epitope.Applications for PRAGMIN Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PRAGMIN
Alternate Names
DKFZp761P0423, homolog of rat pragma of Rnd2, PRAGMIN, sugen kinase 223, tyrosine-protein kinase SgK223
Entrez Gene IDs
157285 (Human)
Gene Symbol
SGK223
Additional PRAGMIN Products
Product Documents for PRAGMIN Antibody
Certificate of Analysis
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Product Specific Notices for PRAGMIN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PRAGMIN Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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