PRAME Antibody (CL5146) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44110

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL5146

Format

Azide and BSA Free
View all PRAME Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P78395, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PRAME Antibody (CL5146) - Azide and BSA Free

PRAME Antibody (CL5146) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PRAME Antibody [NBP3-44110]

Immunocytochemistry/ Immunofluorescence: PRAME Antibody [NBP3-44110]

Staining of U-2 OS cells using the Anti-PRAME monoclonal antibody).
PRAME Antibody (CL5146) - Azide and BSA Free Western Blot: PRAME Antibody [NBP3-44110]

Western Blot: PRAME Antibody [NBP3-44110]

Analysis in human cell line U-2 OS.

Applications for PRAME Antibody (CL5146) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF ixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PRAME

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Alternate Names

cancer/testis antigen 130, MAPEOIP4CT130, melanoma antigen preferentially expressed in tumors, OIP-4, Opa-interacting protein 4, Opa-interacting protein OIP4, preferentially expressed antigen in melanoma, Preferentially expressed antigen of melanoma

Gene Symbol

PRAME

Additional PRAME Products

Product Documents for PRAME Antibody (CL5146) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PRAME Antibody (CL5146) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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