PRAME Antibody (CL5146) - BSA Free

Novus Biologicals | Catalog # NBP2-61148

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL5146

Format

BSA Free
View all PRAME Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P78395, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PRAME Antibody (CL5146) - BSA Free

Western Blot: PRAME Antibody (CL5146) [NBP2-61148]

Western Blot: PRAME Antibody (CL5146) [NBP2-61148]

Western Blot: PRAME Antibody (CL5146) [NBP2-61148] - Analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: PRAME Antibody (CL5146) [NBP2-61148]

Immunocytochemistry/ Immunofluorescence: PRAME Antibody (CL5146) [NBP2-61148]

Immunocytochemistry/Immunofluorescence: PRAME Antibody (CL5146) [NBP2-61148] - Staining of U-2 OS cells using the Anti-PRAME monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Applications for PRAME Antibody (CL5146) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
ICC/IF ixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAME

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Alternate Names

cancer/testis antigen 130, MAPEOIP4CT130, melanoma antigen preferentially expressed in tumors, OIP-4, Opa-interacting protein 4, Opa-interacting protein OIP4, preferentially expressed antigen in melanoma, Preferentially expressed antigen of melanoma

Gene Symbol

PRAME

Additional PRAME Products

Product Documents for PRAME Antibody (CL5146) - BSA Free

Certificate of Analysis

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Product Specific Notices for PRAME Antibody (CL5146) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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