PRMT3 Antibody

Novus Biologicals | Catalog # NBP2-26133

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence DPKTLISEPCGIKH corresponding to internal region according to NP_005779.1, NP_001138638.1, NP_001138639.1.

Specificity

This antibody is expected to recognize all reported isoforms (NP_005779.1; NP_001138638.1; NP_001138639.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PRMT3 Antibody

Western Blot: PRMT3 Antibody [NBP2-26133]

Western Blot: PRMT3 Antibody [NBP2-26133]

Western Blot: PRMT3 Antibody [NBP2-26133] - NBP2-26133 (1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PRMT3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in lysates of MCF7 is reported in scientific literature (PMID: 17439947).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRMT3

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM]

Long Name

Protein arginine N-methyltransferase 3

Alternate Names

EC 2.1.1.319, HRMT1L3

Entrez Gene IDs

10196 (Human)

Gene Symbol

PRMT3

Additional PRMT3 Products

Product Documents for PRMT3 Antibody

Certificate of Analysis

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Product Specific Notices for PRMT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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