Progesterone Antibody (6-5E-10B)

Novus Biologicals | Catalog # NBP2-45210

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Radioimmunoassay

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6-5E-10B
View all Progesterone Primary Antibodies »

Product Specifications

Immunogen

Progesterone- 11a-hemisuccinate conjugated to bovine serum albumin (Pr11a-HMS-BSA)

Localization

Secreted and cytoplasmic

Specificity

This monoclonal antibody is specific for progesterone. It exhibits minimal cross reactivity with related compounds in ELISA. It reacts with Progesterone-11a-HMS-BSA: 100%; 5-beta-Pregnane-3,20-dione: 48%; 5-alpha-Pregnane-3,20-dione: 26.4%; 17-alpha-Hydroxyprogesterone: 2.5% and 20-alpha-Hydroxyprogesterone: 0.04%. Progesterone is a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland. Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Thus, progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Luteinized theca cells of normal ovary secrete progesterone. The determination of progesterone concentrations in the body fluids is of great value for endocrinological investigations in women. This monoclonal antibody may prove useful in identification of ovarian tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

314.46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-53103)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Progesterone Antibody (6-5E-10B)

Immunohistochemistry-Paraffin: Progesterone Antibody (6-5E-10B) [NBP2-45210]

Immunohistochemistry-Paraffin: Progesterone Antibody (6-5E-10B) [NBP2-45210]

Immunohistochemistry-Paraffin: Progesterone Antibody (6-5E-10B) [NBP2-45210] - Mouse cervix tissue stained with Progesterone Antibody. IHC-P image submitted by a verified customer review.
N/A: Progesterone Antibody (6-5E-10B) [NBP2-45210] - Molecular Structure of Progesterone

Applications for Progesterone Antibody (6-5E-10B)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Radioimmunoassay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Not assessed). This Progesterone Antibody (6-5E-10B) is validated for IHC-P from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-45210 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Progesterone

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Alternate Names

4-Pregnen-3,20-dione

Additional Progesterone Products

Product Documents for Progesterone Antibody (6-5E-10B)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Progesterone Antibody (6-5E-10B)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Progesterone Antibody (6-5E-10B)

Customer Reviews for Progesterone Antibody (6-5E-10B) (2)

5 out of 5
2 Customer Ratings
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Customer Images

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  • Progesterone Antibody (6-5E-10B)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse cervix
    Species: Mouse
    Verified Customer | Posted 10/13/2021
    Progesterone Antibody in mouse cervix tissue
    Primary antibody dilution 1:200, incubated at 4C for 10 hours, secondary antibody dilution 1:500
    Progesterone Antibody (6-5E-10B) NBP2-45210
  • Progesterone Antibody (6-5E-10B)
    Name: Anonymous
    Application: ELISA
    Sample Tested: Fecal
    Species: Vertebrate
    Verified Customer | Posted 12/15/2016
    Tested 1:100 Ab conc. Fisher brand 96-well plates with in-house EIA chemicals.
    Elisa assay to test Cortisol in monkey samples
    Progesterone Antibody (6-5E-10B) NBP2-45210

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Protocols

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FAQs

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