Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

Novus Biologicals | Catalog # NB200-320

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 608

Format

Azide Free
View all Progesterone R/NR3C3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH. Accession # P06401

Modification

p Ser294

Localization

Nuclear

Specificity

Specific for endogenous levels of the ~90 kDa Progesterone R/NR3C3-A isoform and the ~120 kDa Progesterone R/NR3C3-B isoform phosphorylated at Ser294. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

Western Blot: Progesterone Receptor [p Ser294] Antibody (608) [NB200-320] - Whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).

Applications for Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000
Application Notes
Recognizes a ~115 kDa doublet/triplet and also a 80 kDa single band in western blots.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Progesterone R/NR3C3

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Long Name

Progesterone Receptor

Alternate Names

NR3C3, PGR, ProgesteroneR

Entrez Gene IDs

5241 (Human)

Gene Symbol

PGR

UniProt

P06401

Additional Progesterone R/NR3C3 Products

Product Documents for Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

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  • Q: I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?

    A:

    We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

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