Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00005034-B01P

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all Protein Disulfide Isomerase/P4HB Primary Antibodies »

Product Specifications

Immunogen

Protein Disulfide Isomerase/P4HB (NP_000909.2, 1 a.a. - 508 a.a.) full-length human protein. MLRRALLCLAVAALVRADAPEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL

Marker

Fibroblast Marker

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0009.jpg
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of purified antibody to P4HB on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in HeLa.
Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in transfected 293T cell line by P4HB polyclonal antibody. Lane 1: P4HB transfected lysate(55.88 KDa). Lane 2: Non-transfected lysate.
Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in human liver.
Protein Disulfide Isomerase/P4HB Antibody

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] -

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Absence of TRIC-B alters expression of collagen-interacting proteins.(A) Quantitative RT-PCR of fibroblast transcripts encoding collagen, modifying enzymes & chaperones. Proband cells (P1, P2, & P3) demonstrate decreased expression of COL1A1 & FKBP10 (FKBP65), & increased expression of PLOD1 (LH1) & HSPA5 (BiP/GRP78) versus control fibroblasts (C). (B) Decreased expression of PLOD2 (LH2), FKBP10 (FKBP65) & PPIB (CyPB) in P2 osteoblasts versus control osteoblasts (C). (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes & chaperones in proband & control cells. Proband fibroblasts show increases in PDI, LH1, LH2 & BiP protein levels. CyPB & FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts & osteoblasts. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1006156), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Applications for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Reviewed Applications

Read 1 review rated 5 using H00005034-B01P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Entrez Gene IDs

5034 (Human)

Gene Symbol

P4HB

UniProt

NP_000909.2 (Human)

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free

Customer Reviews for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MDa-231, Sample Amount: 50ug
    Species: Other
    Verified Customer | Posted 08/06/2010

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Protocols

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