Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03678

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Protein Disulfide Isomerase/P4HB Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human Protein Disulfide Isomerase/P4HB (NP_000909.2). PESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Western Blot: Protein Disulfide Isomerase/P4HB AntibodyBSA Free [NBP3-03678]

Western Blot: Protein Disulfide Isomerase/P4HB AntibodyBSA Free [NBP3-03678]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Analysis of 200ug extracts of SW620 cells using Protein Disulfide Isomerase/P4HB antibody. Western blot was performed from the immunoprecipitate using this antibody.
Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP3-03678] -

Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP3-03678] - Confocal immunofluorescence analysis of U-2OS cells using Protein Disulfide Isomerase/P4HB Rabbit pAb (A0692) at dilution of 1:400. Blue: DAPI for nuclear staining.

Applications for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:100

Immunoprecipitation

1:50-1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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FAQs

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