Protein Phosphatase inhibitor 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37895

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Protein Phosphatase inhibitor 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AESTETQESRPPGIPDTEVESRLGTSGTAKKTAECIPKTHERGSKEPSTKEPSTHIPPLDSKGANSV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Phosphatase inhibitor 1 Antibody - BSA Free

Western Blot: Protein Phosphatase inhibitor 1 Antibody [NBP2-37895]

Western Blot: Protein Phosphatase inhibitor 1 Antibody [NBP2-37895]

Western Blot: Protein Phosphatase inhibitor 1 Antibody [NBP2-37895] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Skeletal muscle tissue
Immunohistochemistry-Paraffin: Protein Phosphatase inhibitor 1 Antibody [NBP2-37895]

Immunohistochemistry-Paraffin: Protein Phosphatase inhibitor 1 Antibody [NBP2-37895]

Immunohistochemistry-Paraffin: Protein Phosphatase inhibitor 1 Antibody [NBP2-37895] - Staining of human kidney shows moderate cytoplasmic positivity in renal tubules.

Applications for Protein Phosphatase inhibitor 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein Phosphatase inhibitor 1

The inhibitor of protein phosphatase 1 (IPP-1, I-1) is an endogenous inhibitor of protein phophastase 1 (PP-1), a signal transducer in many cellular pathways. IPP-1 is a member of heat-stable PP1 inhibitory subunits, which includes DARPP-32, (a homologue of IPP-1) and IPP-2. First discovered in rabbit skeletal muscle, IPP-1 is expressed in various mammalian tissues. IPP-1 inhibition of PP-1 is controlled through phosphorylation at Thr35 by cAMP-dependent protein kinase and a tetra-peptide consensus motif in various PP-1 binding proteins (1). IPP-1 is a hormonal control in muscle contraction, pituitary cell growth, synaptic plasticity by neurotransmitters, and glycogen metabolism (2). In vivo, IPP-1 is phosphorylated at Ser-67 by NCLK, which also initiates inhibition of PP-1 by IPP-1 (3). Triggered by increase in calcium level, IPP-1 is inactivated by PP2B through de-phosphorylation (4).

Alternate Names

I1, I-1, IPP-1, IPP1inhibitor-1, protein phosphatase 1 regulatory subunit 1A, protein phosphatase 1, regulatory (inhibitor) subunit 1A, Protein phosphatase inhibitor 1, protein phosphatase inhibitor-1

Gene Symbol

PPP1R1A

UniProt

Q13522 (Human)

Additional Protein Phosphatase inhibitor 1 Products

Product Documents for Protein Phosphatase inhibitor 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Protein Phosphatase inhibitor 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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