Proteome Profiler Human NFkB Pathway Array
Proteome Profiler Human NFkB Pathway Array Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human NFkB Pathway proteins. Validated for analyte detection in cell lysates.Troubleshooting Guide
- Detects 41 human proteins and 4 serine or tyrosine phosphorylation sites simultaneously
- Requires no specialized equipment
Principle of the Assay
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
Why Use an Antibody Array to Detect Multiple Proteins?
Determining the expression of multiple Proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail, Human NF kappa B Pathway Array
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
Stability and Storage
Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8° C.
|Simultaneously detect the levels of these proteins in a single sample.|
|ASC||IL-18 R alpha||RelA/p65 (pS529)|
|IKK2/IKKβ||NGF R/TNFRSF16||TNF RII/TNFRSF1B|
|IL-1 RI||P53 (pS46)||TRAIL R1/DR4|
|IL-17 RA||RelA/p65||TRAIL R2/DR5|
|Analyte||Quantikine® ELISA Kits||DuoSet® ELISA Development System||DuoSet® IC ELISA Development System (Total)||DuoSet® IC ELISA Development System (Phospho)|
View Larger Image
HCT-116 Untreated vs. Epoxomicin Treated
View Larger Image
Daudi Untreated vs. IFN-alpha 2A Treated
View Larger Image
HT-29 Untreated vs. TNF-alpha +/- MLN4924 Treatment
Preparation and Storage
Citations for Proteome Profiler Human NFkB Pathway Array
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
Cardiac glycosides suppress the maintenance of stemness and malignancy via inhibiting HIF-1? in human glioma stem cells
Authors: DH Lee, S Cheul Oh, AJ Giles, J Jung, MR Gilbert, DM Park
Oncotarget, 2017;8(25):40233-40245. 2017
Adrenomedullin Suppresses Vascular Endothelial Growth Factor-Induced Vascular Hyperpermeability and Inflammation in Retinopathy
Authors: A Imai, Y Toriyama, Y Iesato, K Hirabayash, T Sakurai, A Kamiyoshi, Y Ichikawa-S, H Kawate, M Tanaka, T Liu, X Xian, L Zhai, K Dai, K Tanimura, T Liu, N Cui, A Yamauchi, T Murata, T Shindo
Am. J. Pathol, 2017;0(0):. 2017
Calpain inhibition decreases inflammatory protein expression in vessel walls in a model of chronic myocardial ischemia
Authors: Brittany A Potz
Surgery, 2016;0(0):. 2016
No product specific FAQs exist for this product, however you mayView all Proteome Profiler Antibody Array FAQs
Reviews for Proteome Profiler Human NFkB Pathway Array
There are currently no reviews for this product. Be the first to review Proteome Profiler Human NFkB Pathway Array and earn rewards!
Have you used Proteome Profiler Human NFkB Pathway Array?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image