Proteome Profiler Human NFkB Pathway Array

Contains 4 membranes-each spotted in duplicate with 41 different proteins and 4 serine or tyrosine phosphorylation sites antibodies
Catalog # Availability Size / Price Qty
ARY029

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Detection of human NFkB Pathway proteins in HCT-116 cells.
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Product Details
Citations (8)
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Reviews (4)

Proteome Profiler Human NFkB Pathway Array Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human NFkB Pathway proteins. Validated for analyte detection in cell lysates.

Troubleshooting Guide

Key Benefits

  • Detects 41 human proteins and 4 serine or tyrosine phosphorylation sites simultaneously
  • Requires no specialized equipment
 

 

Principle of the Assay

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

Why Use an Antibody Array to Detect Multiple Proteins?

Determining the expression of multiple Proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail, Human NF kappa B Pathway Array
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8° C.
 

Simultaneously detect the levels of these proteins in a single sample.
ASC IL-18 R alpha RelA/p65 (pS529)
BCL-10 IRAK1 c-Rel
CARD6 IRF5 SHARPIN
CD40/TNFRSF5 IRF8 SOCS-6
dAP1/BIRC2 JNK1/2 STAT1p91
dAP2/BIRC3 JNK2 STAT1 (pY701)
FADD/MORT1 LTBR/TNFRSF3 STAT2
Fas/TNFRSF6/CD95 Metadherin/AEG-1 STAT2 (pY689)
IκBα MYD88 STING/TMEM173
IκBε NFκB1 TLR2
IKK1/IKKα/CHUK NFκB2 TNF RI/TNFRSF1A
IKK2/IKKβ NGF R/TNFRSF16 TNF RII/TNFRSF1B
IKKυ/NEMO P53 TRAF2
IL-1 RI P53 (pS46) TRAIL R1/DR4
IL-17 RA RelA/p65 TRAIL R2/DR5
 

 

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development System DuoSet® IC ELISA Development System (Total) DuoSet® IC ELISA Development System (Phospho)
ASC        
BCL-10        
CARD6        
CD40/TNFRSF5 DCCD40 DY632-05    
dAP1/BIRC2        
dAP2/BIRC3        
FADD/MORT1        
Fas/TNFRSF6/CD95 DFS00 DY326    
IκBα     DYC4299-2  
I&kappaBε        
IKK1/IKKα/CHUK        
IKK2/IKKβ        
IKKΥ/NEMO        
IL-1 RI   DY269    
IL-17 RA   DY177    
IL-18 Rα        
IRAK1        
IRF5        
IRF8        
JNK1/2     DYC1205-2 DYC1387-2
JNK2     DYC1846-2 DYC2236-2
LTBR/TNFRSF3        
Metadherin/AEG-1        
MyD88   DY2928    
NFκB1        
NFκB2        
NGF R/TNFRSF16   DY367    
P53     DYC1043-2  
P53 (pS46)       DYC1489-2
RelΑ/p65        
RelΑ/p65 (pS529)        
c-Rel        
SHARPIN        
SOCS-6        
STAT1p91        
STAT1 (pY701)        
STAT2        
STAT2 (pY689)        
STING/TMEM173        
TLR2 DTLR20 DY2616    
TNF RI/TNFRSF1A DRT100 DY225    
TNF RII/TNFRSF1B DRT200 DY726    
TRAF2        
TRAIL R1/DR4        
TRAIL R2/DR5        

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

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Data Example

Detection of Human NFkB Pathway Proteins in HCT-116 Cell Line. HCT-116 human colorectal carcinoma cells were untreated or treated with 1 µM Epoxomicin (Boston Biochem, Catalog # I-110) for 6 hours (500 µg lysate, 5 minute exposure). RS = Reference Spots.

Detection of Human NFkB Pathway Proteins in Daudi Cell Line. Daudi human Burkitt’s lymphoma cells were untreated or treated with 500 U/mL IFN-alpha 2A (R&D Systems, Catalog # 11100-1) for five minutes (1 mg lysate, 10 minute exposure).

Detection of Human NFkB Pathway Proteins in HT-29 Cell Line. HT-29 human colorectal carcinoma cells were untreated or treated with 50 ng/mL recombinant human TNF-alpha (R&D Systems, Catalog # 210-TA) for ten minutes with or without pretreatment with 5 µM MLN4924 (Boston Biochem, Catalog # I-502) for 1 hour (400 µg lysate, 5 minute exposure).

Citations for Proteome Profiler Human NFkB Pathway Array

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. HMGB1 promotes tumor progression and invasion through HMGB1/TNFR1/NF-&kappaB axis in castration-resistant prostate cancer
    Authors: AR Jung, GE Kim, MY Kim, US Ha, SH Hong, JY Lee, SW Kim, YH Park
    American journal of cancer research, 2021;11(5):2215-2227.  2021
  2. The Effect of Neddylation Inhibition on Inflammation-Induced MMP9 Gene Expression in Esophageal Squamous Cell Carcinoma
    Authors: A Hryniewicz, J Wierzbicki, R Tabola, K Stach, K Sossey-Ala, K Augoff
    International Journal of Molecular Sciences, 2021;22(4):.  2021
  3. Self-assembly of differentiated progenitor cells facilitates spheroid human skin organoid formation and planar skin regeneration
    Authors: P Ebner-Peki, L Krisch, M Wolf, S Hochmann, A Hoog, B Vári, K Muigg, R Poupardin, C Scharler, S Schmidhube, E Russe, H Stachelsch, A Schneeberg, K Schallmose, D Strunk
    Theranostics, 2021;11(17):8430-8447.  2021
  4. TNFa and IL2 Encoding Oncolytic Adenovirus Activates Pathogen and Danger-Associated Immunological Signaling
    Authors: C Heiniö, R Havunen, J Santos, K Lint, V Cervera-Ca, A Kanerva, A Hemminki
    Cells, 2020;9(4):.  2020
  5. Fasciola hepatica GST downregulates NF-?B pathway effectors and inflammatory cytokines while promoting survival in a mouse septic shock model
    Authors: V Aguayo, BN Valdés Fer, M Rodríguez-, C Ruiz-Jimén, MJ Ramos-Bení, LB Méndez, AM Espino
    Sci Rep, 2019;9(1):2275.  2019
  6. Cardiac glycosides suppress the maintenance of stemness and malignancy via inhibiting HIF-1? in human glioma stem cells
    Authors: DH Lee, S Cheul Oh, AJ Giles, J Jung, MR Gilbert, DM Park
    Oncotarget, 2017;8(25):40233-40245.  2017
  7. Adrenomedullin Suppresses Vascular Endothelial Growth Factor-Induced Vascular Hyperpermeability and Inflammation in Retinopathy
    Authors: A Imai, Y Toriyama, Y Iesato, K Hirabayash, T Sakurai, A Kamiyoshi, Y Ichikawa-S, H Kawate, M Tanaka, T Liu, X Xian, L Zhai, K Dai, K Tanimura, T Liu, N Cui, A Yamauchi, T Murata, T Shindo
    Am. J. Pathol, 2017;0(0):.  2017
  8. Calpain inhibition decreases inflammatory protein expression in vessel walls in a model of chronic myocardial ischemia
    Authors: Brittany A Potz
    Surgery, 2016;0(0):.  2016

FAQs

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Reviews for Proteome Profiler Human NFkB Pathway Array

Average Rating: 4.3 (Based on 4 Reviews)

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Proteome Profiler Human NFkB Pathway Array
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Proteome Profiler Human NFkB Pathway Array
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Proteome Profiler Human NFkB Pathway Array
By Anonymous on 08/08/2019

Proteome Profiler Human NFkB Pathway Array
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