Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

Catalog # Availability Size / Price Qty
DYC1387B-5
DYC1387B-2
Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA Data
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Product Details
Citations (6)
FAQs
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Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat JNK in cell lysates. An immobilized capture antibody specific for JNK binds both phosphorylated and unphosphorylated JNK. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*


Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Data Example

Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: JNK

Members of the MAPK family, the c-Jun N-terminal kinases (JNKs) are activated by environmental stresses and inflammatory cytokines. Ten JNK isoforms are created by alternative splicing of mRNA transcripts derived from three genes: JNK1, JNK2, and JNK3. All JNKs are activated by dual phosphorylation; at T183/Y185 for JNK1 and 2, and T221/Y223 for JNK3. Activated JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Long Name:
C-Jun N-terminal Kinase
Alternate Names:
c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8JNK; protein kinase JNK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1

Citations for Human/Mouse/Rat Phospho-JNK Pan Specific DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection
    Authors: Joerg Mattes
    Am. J. Physiol. Lung Cell Mol. Physiol., 2016;0(0):ajplung.00200.
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. Cyclopamine and jervine induce COX-2 overexpression in human erythroleukemia cells but only cyclopamine has a pro-apoptotic effect.
    Authors: Ghezali L, Leger D, Limami Y, Cook-Moreau J, Beneytout J, Liagre B
    Exp Cell Res, 2013;319(7):1043-53.
  3. Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse.
    Authors: Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ
    J. Cell. Biochem., 2010;109(6):1185-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Enhanced IL-1beta-induced IL-8 production in cystic fibrosis lung epithelial cells is dependent of both mitogen-activated protein kinases and NF-kappaB signaling.
    Authors: Muselet-Charlier C, Roque T, Boncoeur E, Chadelat K, Clement A, Jacquot J, Tabary O
    Biochem. Biophys. Res. Commun., 2007;357(2):402-7.
    Species: Human
    Sample Types: Cell Lysates
  5. Low dose leflunomide activates PI3K/Akt signalling in erythroleukemia cells and reduces apoptosis induced by anticancer agents.
    Authors: Leger DY, Liagre B, Beneytout JL
    Apoptosis, 2006;11(10):1747-60.
    Species: Human
    Sample Types: Cell Lysates
  6. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
    Authors: Vergne-Salle</LastName><F P</Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
    Cytokine, 2005;31(5):335-48.
    Species: Human
    Sample Types: Cell Lysates

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