Proteome Profiler Rat Adipokine Array Kit

R&D Systems | Catalog # ARY016

Contains 4 membranes - each spotted in duplicate with 30 different obesity-related antibodies
R&D Systems
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Key Product Details

Species

Rat

Product Summary for Proteome Profiler Rat Adipokine Array Kit

Kit Summary

To simultaneously detect the relative levels of 30 different obesity-related molecules in a single sample. No specialized equipment is necessary.

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided and Other Materials Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 
Simultaneously detect the levels of these obesity-related proteins in a single sample.
ANGPT-L3 IGFBP-1 LIF
CCL2/MCP-1/JE IGFBP-2 Lipocalin-2/NGAL
CCL5/RANTES IGFBP-3 M-CSF
DPPIV/CD26 IGFBP-5 PAI-1/Serpin E1
Endocan IGFBP-6 Pref-1
FGF-21 IL-1 beta RAGE
HGF IL-6 Resistin
ICAM-1 IL-10 TIMP-1
IGF-I IL-11 TNF-alpha
IGF-II Leptin VEGF
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Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for Proteome Profiler Rat Adipokine Array Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Proteome Profiler Rat Adipokine Array Kit

For research use only

Related Research Areas

Citations for Proteome Profiler Rat Adipokine Array Kit

Customer Reviews for Proteome Profiler Rat Adipokine Array Kit (2)

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  • Proteome Profiler Rat Adipokine Array Kit
    Name: saleh almarshad
    Verified Customer | Posted 03/19/2025
    By Saleh Almarshad
    Proteome Profiler Rat Adipokine Array Kit ARY016
  • Proteome Profiler Rat Adipokine Array Kit
    Name: Ivan Luzardo
    Verified Customer | Posted 12/11/2018

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