Kit Summary

To simultaneously detect the relative levels of 30 different obesity-related molecules in a single sample. No specialized equipment is necessary.

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided and Other Materials Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 
Simultaneously detect the levels of these obesity-related proteins in a single sample.
ANGPT-L3 IGFBP-1 LIF
CCL2/MCP-1/JE IGFBP-2 Lipocalin-2/NGAL
CCL5/RANTES IGFBP-3 M-CSF
DPPIV/CD26 IGFBP-5 PAI-1/Serpin E1
Endocan IGFBP-6 Pref-1
FGF-21 IL-1 beta RAGE
HGF IL-6 Resistin
ICAM-1 IL-10 TIMP-1
IGF-I IL-11 TNF-alpha
IGF-II Leptin VEGF
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
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