Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
4 Array Membranes
Detection Antibody Cocktail
Chemiluminescent Detection Reagents
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For a complete list of the kit contents and necessary materials, please see the Materials Provided and Other Materials Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these obesity-related proteins in a single sample.
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