Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2B3

Format

Azide and BSA Free
View all Protogenin Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRTG (NP_776175) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

126.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Protogenin Antibody (OTI2B3) - Azide and BSA Free (NBP2-02745) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Protogenin Antibody (OTI2B3) - Azide and BSA Free

Western Blot: Protogenin Antibody (OTI2B3)Azide and BSA Free [NBP2-73674]

Western Blot: Protogenin Antibody (OTI2B3)Azide and BSA Free [NBP2-73674]

Western Blot: Protogenin Antibody (OTI2B3) - Azide and BSA Free [NBP2-73674] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protogenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protogenin.
Immunocytochemistry/ Immunofluorescence: Protogenin Antibody (OTI2B3) - Azide and BSA Free [NBP2-73674]

Immunocytochemistry/ Immunofluorescence: Protogenin Antibody (OTI2B3) - Azide and BSA Free [NBP2-73674]

Immunocytochemistry/Immunofluorescence: Protogenin Antibody (OTI2B3) - Azide and BSA Free [NBP2-73674] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PRTG.

Applications for Protogenin Antibody (OTI2B3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protogenin

PRTG may play a role in anteroposterior axis elongation

Alternate Names

IGDCC5, PRTG, RPTG, Shen-Dan

Gene Symbol

PRTG

Additional Protogenin Products

Product Documents for Protogenin Antibody (OTI2B3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Protogenin Antibody (OTI2B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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