PRPF3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-19114

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PRPF3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human U4/U6 small nuclear ribonucleoprotein Prp3 using the numbering given in entry NP_004689.1 (GeneID 9129).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF3 Antibody - BSA Free

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - Detection of mouse hPrp3p by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (NBP1-19114) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-hPrp3p used at a dilution of 1:100.
Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - FFPE section of mouse teratoma. Affinity purified rabbit anti-hPrp3p used at a dilution of 1:100.
Immunohistochemistry: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry: PRPF3 Antibody [NBP1-19114] - Sample: FFPE section of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-hPrp3p used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114]

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - Detection of human hPrp3p by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (NBP1-19114) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: PRPF3 Antibody [NBP1-19114]

Immunoprecipitation: PRPF3 Antibody [NBP1-19114]

Immunoprecipitation: PRPF3 Antibody [NBP1-19114] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-19114 used for IP at 3 mcg/mg lysate. hPrp3p was also immunoprecipitated by NBP1-19115 which recognizes a downstream epitope

Applications for PRPF3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRPF3

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq]

Alternate Names

hPrp3, HPRP3P, HPRP3retinitis pigmentosa 18 (autosomal dominant), Pre-mRNA-splicing factor 3, Prp3, PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae), PRP3 pre-mRNA processing factor 3 homolog (yeast), Prp3p, RP18, U4/U6 small nuclear ribonucleoprotein Prp3, U4/U6 snRNP 90 kDa protein, U4/U6-associated RNA splicing factor

Entrez Gene IDs

9129 (Human)

Gene Symbol

PRPF3

UniProt

NP_004689.1 (Human)

Additional PRPF3 Products

Product Documents for PRPF3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PRPF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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