Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Orangutan (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PRPF38A Primary Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 262 to 312 of human Pre-mRNA Processing Factor 38A using the numbering given in entry NP_116253.2 (GeneID 84950).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF38A Antibody - BSA Free

Western Blot: PRPF38A Antibody [NBP2-37697]

Western Blot: PRPF38A Antibody [NBP2-37697]

Western Blot: PRPF38A Antibody [NBP2-37697] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PRPF38A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PRPF38A Antibody [NBP2-37697]

Immunoprecipitation: PRPF38A Antibody [NBP2-37697]

Immunoprecipitation: PRPF38A Antibody [NBP2-37697] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PRPF38A antibody used for IP at 6 ug per reaction. PRPF38A was also immunoprecipitated by rabbit anti-PRPF38A antibody. For blotting immunoprecipitated PRPF38A, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PRPF38A Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRPF38A

Alternate Names

FLJ14936, MGC3320, pre-mRNA-splicing factor 38A, Prp38, PRP38 pre-mRNA processing factor 38 (yeast) domain containing A, RP5-965L7.1

Gene Symbol

PRPF38A

UniProt

NP_116253.2 (Human)

Additional PRPF38A Products

Product Documents for PRPF38A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PRPF38A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PRPF38A Antibody - BSA Free

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