PRPF6 Antibody

Novus Biologicals | Catalog # NBP1-46212

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1 and 50 of human Pre-mRNA Processing Factor 6 using the numbering given in entry NP_036601.2 (GeneID 24148).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF6 Antibody

Western Blot: PRPF6 Antibody [NBP1-46212]

Western Blot: PRPF6 Antibody [NBP1-46212]

Western Blot: PRPF6 Antibody [NBP1-46212] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PRP6 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody NBP1-46213, which recognizes a downstream epitope.
Immunohistochemistry: PRPF6 Antibody [NBP1-46212]

Immunohistochemistry: PRPF6 Antibody [NBP1-46212]

Immunohistochemistry: PRPF6 Antibody [NBP1-46212] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for PRPF6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:1,000

Immunohistochemistry-Paraffin

1:100 to 1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRPF6

The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.

Alternate Names

androgen receptor N-terminal domain transactivating protein-1, ANT-1, bB152O15.1, C20orf14, chromosome 20 open reading frame 14, hPrp6, p102 U5 small nuclear ribonucleoprotein particle-binding protein, pre-mRNA-processing factor 6, Prp6, PRP6 homolog, PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae), PRP6 pre-mRNA processing factor 6 homolog (yeast), putative mitochondrial outer membrane protein import receptor, TOM, U5 snRNP-associated 102 kDa protein, U5-102 kDa protein, U5-102K

Gene Symbol

PRPF6

Additional PRPF6 Products

Product Documents for PRPF6 Antibody

Certificate of Analysis

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Product Specific Notices for PRPF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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